Crystallization and preliminary X-ray studies of a Bacillus subtilis and Thermus thermophilus HB8 chimeric 3-isopropylmalate dehydrogenase and thermostable mutants of it

Masahiro Sakurai, Ko Onodera, Hideaki Moriyama, Osamau Matsumoto, Nobuo Tanaka, Koichi Numata, Katsumi Imada, Mamoru Sato, Yukiteru Katsube, Tairo Oshima

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Abstract

A new type of chimeric 3-isopropylmalate dehydrogenase (2T2M6T) was produced by expressing the fused gene of Bacillus subtilis and Thermus thermophilus. The enzyme shows heat stability intermediate between those of the parents. The crystal of the enzyme belongs to the space group of P3221, with cell dimensions of a= 6=78.9 A and c = 158.9 A. Two thermostable mutants of the chimeric enzyme were prepared by site-directed mutagenesis and then crystallized.

Original languageEnglish (US)
Pages (from-to)173-174
Number of pages2
JournalJournal of Biochemistry
Volume112
Issue number2
DOIs
StatePublished - Aug 1992

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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