Crystallization and preliminary X-ray analyses of quaternary, ternary and binary protein - DNA complexes with involvement of AML1/Runx-1/CBFα Runt domain, CBFβ and the C/EBPβ bZip region

Tahir H Tahirov, T. Inoue-Bungo, M. Sasaki, M. Shiina, K. Kimura, K. Sato, T. Kumasaka, M. Yamamoto, N. Kamiya, K. Ogata

Research output: Contribution to journalArticle

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Abstract

Three types of protein - DNA complexes, AML1/Runx-1/ CBFα(Runt) - CBFβ - C/EBPβ(bZip) - DNA (CBFα-β-C/EBPβ-DNA), AML1/Runx-1/CBFα(Runt) - C/EBPβ(bZip) - DNA (CBFα-C/EBPβ-DNA) and AML1/Runx-1/CBFα(Runt) - DNA (CBFα-DNA), were crystallized. The crystals were all orthorhombic and belonged to space groups C2221, P21212 and P212121, respectively. The resolutions of CBFα-β-C/EBPβ-DNA and CBFα-C/EBPβ-DNA crystals were both 3 Å, while that of the CBFα-DNA crystal was 2.65 Å. Complete data sets were collected for all of the native crystals, along with MAD and MIR data sets for CBFα-β-C/EBPβ-DNA. The heavy-atom site was determined using MAD data for a gold derivative of CBFα-β-C/EBPβ-DNA.

Original languageEnglish (US)
Pages (from-to)850-853
Number of pages4
JournalActa Crystallographica Section D: Biological Crystallography
Volume57
Issue number6
DOIs
StatePublished - Jul 4 2001

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Crystallization
deoxyribonucleic acid
X-Rays
crystallization
proteins
X rays
DNA
Proteins
x rays
Crystals
crystals
Gold
gold
Derivatives
Atoms

ASJC Scopus subject areas

  • Structural Biology

Cite this

Crystallization and preliminary X-ray analyses of quaternary, ternary and binary protein - DNA complexes with involvement of AML1/Runx-1/CBFα Runt domain, CBFβ and the C/EBPβ bZip region. / Tahirov, Tahir H; Inoue-Bungo, T.; Sasaki, M.; Shiina, M.; Kimura, K.; Sato, K.; Kumasaka, T.; Yamamoto, M.; Kamiya, N.; Ogata, K.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 57, No. 6, 04.07.2001, p. 850-853.

Research output: Contribution to journalArticle

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abstract = "Three types of protein - DNA complexes, AML1/Runx-1/ CBFα(Runt) - CBFβ - C/EBPβ(bZip) - DNA (CBFα-β-C/EBPβ-DNA), AML1/Runx-1/CBFα(Runt) - C/EBPβ(bZip) - DNA (CBFα-C/EBPβ-DNA) and AML1/Runx-1/CBFα(Runt) - DNA (CBFα-DNA), were crystallized. The crystals were all orthorhombic and belonged to space groups C2221, P21212 and P212121, respectively. The resolutions of CBFα-β-C/EBPβ-DNA and CBFα-C/EBPβ-DNA crystals were both 3 {\AA}, while that of the CBFα-DNA crystal was 2.65 {\AA}. Complete data sets were collected for all of the native crystals, along with MAD and MIR data sets for CBFα-β-C/EBPβ-DNA. The heavy-atom site was determined using MAD data for a gold derivative of CBFα-β-C/EBPβ-DNA.",
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AU - Tahirov, Tahir H

AU - Inoue-Bungo, T.

AU - Sasaki, M.

AU - Shiina, M.

AU - Kimura, K.

AU - Sato, K.

AU - Kumasaka, T.

AU - Yamamoto, M.

AU - Kamiya, N.

AU - Ogata, K.

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