Crystallization and preliminary crystallographic analysis of the nickel-responsive regulator NikR from Pyrococcus horikoshii

Tomoe Kitao, Chizu Kuroishi, Tahir H. Tahirov

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The nickel-responsive repressor from Pyrococcus horikoshii OT3 (PhNikR) has been crystallized in the apo form (PhNikR-apo) and two nickel-bound forms (PhNikR-Ni-1 and PhNikR-Ni-2). The PhNikR-apo crystals belong to space group P21, with unit-cell parameters a = 75.78, b = 54.32, c = 77.28 Å, β = 116.07°, and diffract to 2.2 Å. The PhNikR-Ni-1 crystals belong to space group P41212, with unit-cell parameters a = b = 99.89, c = 97.98 Å, and diffract to 3.0 Å and the PhNikR-Ni-2 crystals belong to space group P3121 or P3221, with unit-cell parameters a = b = 109.95, c = 79.0 Å, and diffract to 2.1 Å. The crystals obtained were suitable for detailed structural studies.

Original languageEnglish (US)
Pages (from-to)43-45
Number of pages3
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume61
Issue number1
DOIs
StatePublished - Dec 1 2005

Fingerprint

Pyrococcus horikoshii
regulators
Crystallization
Nickel
nickel
crystallization
Crystals
crystals
cells

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

Cite this

@article{8739c1d976984d0aae1780e6cb53ba0d,
title = "Crystallization and preliminary crystallographic analysis of the nickel-responsive regulator NikR from Pyrococcus horikoshii",
abstract = "The nickel-responsive repressor from Pyrococcus horikoshii OT3 (PhNikR) has been crystallized in the apo form (PhNikR-apo) and two nickel-bound forms (PhNikR-Ni-1 and PhNikR-Ni-2). The PhNikR-apo crystals belong to space group P21, with unit-cell parameters a = 75.78, b = 54.32, c = 77.28 {\AA}, β = 116.07°, and diffract to 2.2 {\AA}. The PhNikR-Ni-1 crystals belong to space group P41212, with unit-cell parameters a = b = 99.89, c = 97.98 {\AA}, and diffract to 3.0 {\AA} and the PhNikR-Ni-2 crystals belong to space group P3121 or P3221, with unit-cell parameters a = b = 109.95, c = 79.0 {\AA}, and diffract to 2.1 {\AA}. The crystals obtained were suitable for detailed structural studies.",
author = "Tomoe Kitao and Chizu Kuroishi and Tahirov, {Tahir H.}",
year = "2005",
month = "12",
day = "1",
doi = "10.1107/S1744309104025473",
language = "English (US)",
volume = "61",
pages = "43--45",
journal = "Acta Crystallographica Section F:Structural Biology Communications",
issn = "1744-3091",
publisher = "John Wiley and Sons Ltd",
number = "1",

}

TY - JOUR

T1 - Crystallization and preliminary crystallographic analysis of the nickel-responsive regulator NikR from Pyrococcus horikoshii

AU - Kitao, Tomoe

AU - Kuroishi, Chizu

AU - Tahirov, Tahir H.

PY - 2005/12/1

Y1 - 2005/12/1

N2 - The nickel-responsive repressor from Pyrococcus horikoshii OT3 (PhNikR) has been crystallized in the apo form (PhNikR-apo) and two nickel-bound forms (PhNikR-Ni-1 and PhNikR-Ni-2). The PhNikR-apo crystals belong to space group P21, with unit-cell parameters a = 75.78, b = 54.32, c = 77.28 Å, β = 116.07°, and diffract to 2.2 Å. The PhNikR-Ni-1 crystals belong to space group P41212, with unit-cell parameters a = b = 99.89, c = 97.98 Å, and diffract to 3.0 Å and the PhNikR-Ni-2 crystals belong to space group P3121 or P3221, with unit-cell parameters a = b = 109.95, c = 79.0 Å, and diffract to 2.1 Å. The crystals obtained were suitable for detailed structural studies.

AB - The nickel-responsive repressor from Pyrococcus horikoshii OT3 (PhNikR) has been crystallized in the apo form (PhNikR-apo) and two nickel-bound forms (PhNikR-Ni-1 and PhNikR-Ni-2). The PhNikR-apo crystals belong to space group P21, with unit-cell parameters a = 75.78, b = 54.32, c = 77.28 Å, β = 116.07°, and diffract to 2.2 Å. The PhNikR-Ni-1 crystals belong to space group P41212, with unit-cell parameters a = b = 99.89, c = 97.98 Å, and diffract to 3.0 Å and the PhNikR-Ni-2 crystals belong to space group P3121 or P3221, with unit-cell parameters a = b = 109.95, c = 79.0 Å, and diffract to 2.1 Å. The crystals obtained were suitable for detailed structural studies.

UR - http://www.scopus.com/inward/record.url?scp=17144376072&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=17144376072&partnerID=8YFLogxK

U2 - 10.1107/S1744309104025473

DO - 10.1107/S1744309104025473

M3 - Article

C2 - 16508086

AN - SCOPUS:17144376072

VL - 61

SP - 43

EP - 45

JO - Acta Crystallographica Section F:Structural Biology Communications

JF - Acta Crystallographica Section F:Structural Biology Communications

SN - 1744-3091

IS - 1

ER -