Crystallization and preliminary crystallographic analysis of the complex of the second and third regulatory subunits of human Pol δ

Andrey G. Baranovskiy, Nigar D. Babayeva, Youri I Pavlov, Tahir H Tahirov

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Human DNA polymerase δ (Pol δ) consists of four subunits: p125, p50, p66 and p12. A heterodimer containing a His-tagged p50 subunit (p50) and a p50-interacting domain of the p66 subunit (p66N) was crystallized. The crystal was in the form of a prism with a rhombic cross-section and belonged to space group P21. The crystal had unit-cell parameters a = 95.13, b = 248.54, c = 103.46 Å, β = 106.94° and diffracted to a resolution of 3 Å. Four molecules of p50-p66N in an asymmetric unit corresponded to a crystal solvent content of 72.2%.

Original languageEnglish (US)
Pages (from-to)822-824
Number of pages3
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume64
Issue number9
DOIs
StatePublished - Sep 11 2008

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DNA-Directed DNA Polymerase
Crystallization
crystallization
Crystals
crystals
Prisms
prisms
deoxyribonucleic acid
Molecules
cross sections
cells
molecules

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

Cite this

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title = "Crystallization and preliminary crystallographic analysis of the complex of the second and third regulatory subunits of human Pol δ",
abstract = "Human DNA polymerase δ (Pol δ) consists of four subunits: p125, p50, p66 and p12. A heterodimer containing a His-tagged p50 subunit (p50) and a p50-interacting domain of the p66 subunit (p66N) was crystallized. The crystal was in the form of a prism with a rhombic cross-section and belonged to space group P21. The crystal had unit-cell parameters a = 95.13, b = 248.54, c = 103.46 {\AA}, β = 106.94° and diffracted to a resolution of 3 {\AA}. Four molecules of p50-p66N in an asymmetric unit corresponded to a crystal solvent content of 72.2{\%}.",
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T1 - Crystallization and preliminary crystallographic analysis of the complex of the second and third regulatory subunits of human Pol δ

AU - Baranovskiy, Andrey G.

AU - Babayeva, Nigar D.

AU - Pavlov, Youri I

AU - Tahirov, Tahir H

PY - 2008/9/11

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AB - Human DNA polymerase δ (Pol δ) consists of four subunits: p125, p50, p66 and p12. A heterodimer containing a His-tagged p50 subunit (p50) and a p50-interacting domain of the p66 subunit (p66N) was crystallized. The crystal was in the form of a prism with a rhombic cross-section and belonged to space group P21. The crystal had unit-cell parameters a = 95.13, b = 248.54, c = 103.46 Å, β = 106.94° and diffracted to a resolution of 3 Å. Four molecules of p50-p66N in an asymmetric unit corresponded to a crystal solvent content of 72.2%.

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