Crystallization and preliminary crystallographic analysis of T7 RNA polymerase elongation complex

Dmitry Temiakov, Tahir H Tahirov, Michael Anikin, William T. McAllister, Dmitry G. Vassylyev, Shigeyuki Yokoyama

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A phage T7 RNA polymerase elongation complex containing 8 bp of RNA-DNA hybrid and 10 bp of downstream DNA with a total molecular mass of ∼110 kDa was crystallized. A complete diffraction data set was collected at 2.6 Å resolution. The structure determination and refinement are in progress.

Original languageEnglish (US)
Pages (from-to)185-187
Number of pages3
JournalActa Crystallographica - Section D Biological Crystallography
Volume59
Issue number1
DOIs
StatePublished - Jan 1 2003

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Crystallization
elongation
Elongation
deoxyribonucleic acid
crystallization
Bacteriophage T7
Bacteriophages
DNA
Molecular mass
Diffraction
RNA
diffraction
bacteriophage T7 RNA polymerase
Datasets

ASJC Scopus subject areas

  • Structural Biology

Cite this

Crystallization and preliminary crystallographic analysis of T7 RNA polymerase elongation complex. / Temiakov, Dmitry; Tahirov, Tahir H; Anikin, Michael; McAllister, William T.; Vassylyev, Dmitry G.; Yokoyama, Shigeyuki.

In: Acta Crystallographica - Section D Biological Crystallography, Vol. 59, No. 1, 01.01.2003, p. 185-187.

Research output: Contribution to journalArticle

Temiakov, Dmitry ; Tahirov, Tahir H ; Anikin, Michael ; McAllister, William T. ; Vassylyev, Dmitry G. ; Yokoyama, Shigeyuki. / Crystallization and preliminary crystallographic analysis of T7 RNA polymerase elongation complex. In: Acta Crystallographica - Section D Biological Crystallography. 2003 ; Vol. 59, No. 1. pp. 185-187.
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