Crystallization and initial X-ray analysis of alkaline xylanase

Ning Lu, Hideaki Moriyama, Satoshi Nakamura, Takao Sato, Nobuo Tanaka

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Crystals of alkaline xylanase (xylanase J) from alkaliphilic Bacillus sp. 41M-1 were grown by decreasing the temperature of the protein solution. Initial X-ray analysis of the crystal showed a tetragonal system with space group P41 or P43 and unit-cell parameters a = b = 115.7, c = 46.0 Å. Assuming two molecules per asymmetric unit, V(m) = 2.31 Å3 Da-1. The crystals diffract X-rays to 2.6 Å resolution at 100 K.

Original languageEnglish (US)
Pages (from-to)464-465
Number of pages2
JournalActa Crystallographica Section D: Biological Crystallography
Volume56
Issue number4
DOIs
StatePublished - May 11 2000

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X ray analysis
Crystallization
X-Rays
crystallization
Endo-1,4-beta Xylanases
Crystals
Bacillus
crystals
x rays
Bacilli
Temperature
proteins
X rays
Proteins
Molecules
cells
molecules
temperature

ASJC Scopus subject areas

  • Structural Biology

Cite this

Crystallization and initial X-ray analysis of alkaline xylanase. / Lu, Ning; Moriyama, Hideaki; Nakamura, Satoshi; Sato, Takao; Tanaka, Nobuo.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 56, No. 4, 11.05.2000, p. 464-465.

Research output: Contribution to journalArticle

Lu, Ning ; Moriyama, Hideaki ; Nakamura, Satoshi ; Sato, Takao ; Tanaka, Nobuo. / Crystallization and initial X-ray analysis of alkaline xylanase. In: Acta Crystallographica Section D: Biological Crystallography. 2000 ; Vol. 56, No. 4. pp. 464-465.
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