Crystallization and initial X-ray analysis of alkaline xylanase

Ning Lu, Hideaki Moriyama, Satoshi Nakamura, Takao Sato, Nobuo Tanaka

Research output: Contribution to journalArticle

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Abstract

Crystals of alkaline xylanase (xylanase J) from alkaliphilic Bacillus sp. 41M-1 were grown by decreasing the temperature of the protein solution. Initial X-ray analysis of the crystal showed a tetragonal system with space group P41 or P43 and unit-cell parameters a = b = 115.7, c = 46.0 Å. Assuming two molecules per asymmetric unit, V(m) = 2.31 Å3 Da-1. The crystals diffract X-rays to 2.6 Å resolution at 100 K.

Original languageEnglish (US)
Pages (from-to)464-465
Number of pages2
JournalActa Crystallographica Section D: Biological Crystallography
Volume56
Issue number4
DOIs
Publication statusPublished - May 11 2000

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ASJC Scopus subject areas

  • Structural Biology

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