Crystallization and avoiding the problem of hemihedral twinning in crystals of Δ1-pyrroline-5-carboxylate dehydrogenase from Thermus thermophilus

Eiji Inagaki, Hitomi Takahashi, Chizu Kuroishi, Tahir H. Tahirov

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Δ1-Pyrroline-5-carboxylate dehydrogenase from Thermus thermophilus (TtP5CDh) has been crystallized in a citrate-bound form (TtP5CDh-cit). The crystals diffracted to well beyond 2 Å resolution, but exhibited perfect or near-perfect hemihedral twinning. Variation of crystallization conditions resulted in the growth of larger untwinned crystals or crystals with significantly reduced twin content, all with similar unit-cell parameters. The soaking of TtP5CDh-cit crystals in citrate-free solution produced crystals of the apo form (TtP5CDh-apo). The TtP5CDh-apo crystals belong to space group R3, with unit-cell parameters a = b = 102.29, c = 279.28 Å, and diffract to 1.08 Å. Crystals soaked in solution with NAD + (TtP5CDh-NAD), NADH (TtP5CDh-NADH) and glutamate (TtP5CDh-Glu) were also prepared and characterized.

Original languageEnglish (US)
Pages (from-to)609-611
Number of pages3
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume61
Issue number6
DOIs
StatePublished - Dec 1 2005

Fingerprint

1-Pyrroline-5-Carboxylate Dehydrogenase
Thermus thermophilus
dehydrogenases
Twinning
twinning
Crystallization
NAD
carboxylates
crystallization
Citric Acid
Crystals
crystals
citrates
Glutamic Acid
glutamates
Growth
soaking
cells

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

Cite this

@article{c7a80e362411463d8bba120763b7000f,
title = "Crystallization and avoiding the problem of hemihedral twinning in crystals of Δ1-pyrroline-5-carboxylate dehydrogenase from Thermus thermophilus",
abstract = "Δ1-Pyrroline-5-carboxylate dehydrogenase from Thermus thermophilus (TtP5CDh) has been crystallized in a citrate-bound form (TtP5CDh-cit). The crystals diffracted to well beyond 2 {\AA} resolution, but exhibited perfect or near-perfect hemihedral twinning. Variation of crystallization conditions resulted in the growth of larger untwinned crystals or crystals with significantly reduced twin content, all with similar unit-cell parameters. The soaking of TtP5CDh-cit crystals in citrate-free solution produced crystals of the apo form (TtP5CDh-apo). The TtP5CDh-apo crystals belong to space group R3, with unit-cell parameters a = b = 102.29, c = 279.28 {\AA}, and diffract to 1.08 {\AA}. Crystals soaked in solution with NAD + (TtP5CDh-NAD), NADH (TtP5CDh-NADH) and glutamate (TtP5CDh-Glu) were also prepared and characterized.",
author = "Eiji Inagaki and Hitomi Takahashi and Chizu Kuroishi and Tahirov, {Tahir H.}",
year = "2005",
month = "12",
day = "1",
doi = "10.1107/S1744309105016118",
language = "English (US)",
volume = "61",
pages = "609--611",
journal = "Acta Crystallographica Section F:Structural Biology Communications",
issn = "1744-3091",
publisher = "John Wiley and Sons Ltd",
number = "6",

}

TY - JOUR

T1 - Crystallization and avoiding the problem of hemihedral twinning in crystals of Δ1-pyrroline-5-carboxylate dehydrogenase from Thermus thermophilus

AU - Inagaki, Eiji

AU - Takahashi, Hitomi

AU - Kuroishi, Chizu

AU - Tahirov, Tahir H.

PY - 2005/12/1

Y1 - 2005/12/1

N2 - Δ1-Pyrroline-5-carboxylate dehydrogenase from Thermus thermophilus (TtP5CDh) has been crystallized in a citrate-bound form (TtP5CDh-cit). The crystals diffracted to well beyond 2 Å resolution, but exhibited perfect or near-perfect hemihedral twinning. Variation of crystallization conditions resulted in the growth of larger untwinned crystals or crystals with significantly reduced twin content, all with similar unit-cell parameters. The soaking of TtP5CDh-cit crystals in citrate-free solution produced crystals of the apo form (TtP5CDh-apo). The TtP5CDh-apo crystals belong to space group R3, with unit-cell parameters a = b = 102.29, c = 279.28 Å, and diffract to 1.08 Å. Crystals soaked in solution with NAD + (TtP5CDh-NAD), NADH (TtP5CDh-NADH) and glutamate (TtP5CDh-Glu) were also prepared and characterized.

AB - Δ1-Pyrroline-5-carboxylate dehydrogenase from Thermus thermophilus (TtP5CDh) has been crystallized in a citrate-bound form (TtP5CDh-cit). The crystals diffracted to well beyond 2 Å resolution, but exhibited perfect or near-perfect hemihedral twinning. Variation of crystallization conditions resulted in the growth of larger untwinned crystals or crystals with significantly reduced twin content, all with similar unit-cell parameters. The soaking of TtP5CDh-cit crystals in citrate-free solution produced crystals of the apo form (TtP5CDh-apo). The TtP5CDh-apo crystals belong to space group R3, with unit-cell parameters a = b = 102.29, c = 279.28 Å, and diffract to 1.08 Å. Crystals soaked in solution with NAD + (TtP5CDh-NAD), NADH (TtP5CDh-NADH) and glutamate (TtP5CDh-Glu) were also prepared and characterized.

UR - http://www.scopus.com/inward/record.url?scp=33744497047&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33744497047&partnerID=8YFLogxK

U2 - 10.1107/S1744309105016118

DO - 10.1107/S1744309105016118

M3 - Article

C2 - 16511109

AN - SCOPUS:33744497047

VL - 61

SP - 609

EP - 611

JO - Acta Crystallographica Section F:Structural Biology Communications

JF - Acta Crystallographica Section F:Structural Biology Communications

SN - 1744-3091

IS - 6

ER -