Crystal Structures of Cystathionine β-Synthase from Saccharomyces cerevisiae: One Enzymatic Step at a Time

Yupeng Tu, Cheryl A. Kreinbring, Megan Hill, Cynthia Liu, Gregory A. Petsko, Christopher D. McCune, David B Berkowitz, Dali Liu, Dagmar Ringe

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3 Scopus citations

Abstract

Cystathionine β-synthase (CBS) is a key regulator of sulfur amino acid metabolism, taking homocysteine from the methionine cycle to the biosynthesis of cysteine via the trans-sulfuration pathway. CBS is also a predominant source of H2S biogenesis. Roles for CBS have been reported for neuronal death pursuant to cerebral ischemia, promoting ovarian tumor growth, and maintaining drug-resistant phenotype by controlling redox behavior and regulating mitochondrial bioenergetics. The trans-sulfuration pathway is well-conserved in eukaryotes, but the analogous enzymes have different enzymatic behavior in different organisms. CBSs from the higher organisms contain a heme in an N-terminal domain. Though the presence of the heme, whose functions in CBSs have yet to be elucidated, is biochemically interesting, it hampers UV-vis absorption spectroscopy investigations of pyridoxal 5′-phosphate (PLP) species. CBS from Saccharomyces cerevisiae (yCBS) naturally lacks the heme-containing N-terminal domain, which makes it an ideal model for spectroscopic studies of the enzymological reaction catalyzed and allows structural studies of the basic yCBS catalytic core (yCBS-cc). Here we present the crystal structure of yCBS-cc, solved to 1.5 Å. Crystal structures of yCBS-cc in complex with enzymatic reaction intermediates have been captured, providing a structural basis for residues involved in catalysis. Finally, the structure of the yCBS-cc cofactor complex generated by incubation with an inhibitor shows apparent off-pathway chemistry not normally seen with CBS.

Original languageEnglish (US)
Pages (from-to)3134-3145
Number of pages12
JournalBiochemistry
Volume57
Issue number22
DOIs
Publication statusPublished - Jun 5 2018

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ASJC Scopus subject areas

  • Biochemistry

Cite this

Tu, Y., Kreinbring, C. A., Hill, M., Liu, C., Petsko, G. A., McCune, C. D., ... Ringe, D. (2018). Crystal Structures of Cystathionine β-Synthase from Saccharomyces cerevisiae: One Enzymatic Step at a Time. Biochemistry, 57(22), 3134-3145. https://doi.org/10.1021/acs.biochem.8b00092