Crystal structure of the long-chain fatty acid transporter FadL

Bert Van Den Berg, Paul N Black, William M. Clemons, Tom A. Rapoport

Research output: Contribution to journalArticle

133 Citations (Scopus)

Abstract

The mechanisms by which hydrophobic molecules, such as long-chain fatty acids, enter cells are poorly understood. In Gram-negative bacteria, the lipopolysaccharide layer in the outer membrane is an efficient barrier for fatty acids and aromatic hydrocarbons destined for biodegradation. We report crystal structures of the long-chain fatty acid transporter FadL from Escherichia coli at 2.6 and 2.8 angstrom resolution.. FadL forms a 14-stranded β barrel that is occluded by a central hatch domain. The structures suggest, that hydrophobic compounds bind to multiple sites in FadL and use a transport mechanism that involves spontaneous conformational changes in the hatch.

Original languageEnglish (US)
Pages (from-to)1506-1509
Number of pages4
JournalScience
Volume304
Issue number5676
DOIs
StatePublished - Jun 4 2004

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Fatty Acids
Aromatic Hydrocarbons
Gram-Negative Bacteria
Lipopolysaccharides
Escherichia coli
Membranes

ASJC Scopus subject areas

  • Medicine(all)
  • General

Cite this

Van Den Berg, B., Black, P. N., Clemons, W. M., & Rapoport, T. A. (2004). Crystal structure of the long-chain fatty acid transporter FadL. Science, 304(5676), 1506-1509. https://doi.org/10.1126/science.1097524

Crystal structure of the long-chain fatty acid transporter FadL. / Van Den Berg, Bert; Black, Paul N; Clemons, William M.; Rapoport, Tom A.

In: Science, Vol. 304, No. 5676, 04.06.2004, p. 1506-1509.

Research output: Contribution to journalArticle

Van Den Berg, B, Black, PN, Clemons, WM & Rapoport, TA 2004, 'Crystal structure of the long-chain fatty acid transporter FadL', Science, vol. 304, no. 5676, pp. 1506-1509. https://doi.org/10.1126/science.1097524
Van Den Berg, Bert ; Black, Paul N ; Clemons, William M. ; Rapoport, Tom A. / Crystal structure of the long-chain fatty acid transporter FadL. In: Science. 2004 ; Vol. 304, No. 5676. pp. 1506-1509.
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