Crystal structure of H2-proteinase from the venom of Trimeresurus flavoviridis

Takashi Kumasaka, Masaki Yamamoto, Hideaki Moriyama, Nobuo Tanaka, Mamoru Sato, Yukiteru Katsube, Yoshio Yamakawa, Tamotsu Omori-Satoh, Sadaaki Iwanaga, Tatzuo Ueki

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

The crystal structure of the zinc-protease, H2-proteinase. isolated from the venom of Trimeresurus flavoviridis has been determined. The crystallographic R factor is 0.176 for 10.635 reflections with F(obs)>2σ(F(obs)) in the 8.0 to 2.2 Å resolution range. The enzyme has two domains with a cleft in which a catalytic zinc atom is located. The N-terminal domain is composed of four helices around a central five-stranded β-sheet. The irregularly folded C-terminal domain contains one helix and two disulfide bridges. These two domains are linked by a disulfide bridge. In the zinc environment, the catalytic zinc atom forms a distorted tetrahedral coordination with three histidines and one catalytic water molecule, and the methionine-containing turn is structurally conserved. These are distinctive features of the metzincins, one of the zinc metalloprotease superfamilies. The entire structure shows good agreement with that of two Crotalus snake venom proteases, adamalysin II and atrolysin C. The H2-proteinase, however, contains no structural calcium ions, and differences of disulfide configurations and the coordination of the catalytic water molecule exist as compared with the other two proteases.

Original languageEnglish (US)
Pages (from-to)49-57
Number of pages9
JournalJournal of Biochemistry
Volume119
Issue number1
DOIs
StatePublished - Jan 1 1996
Externally publishedYes

Fingerprint

Venoms
Zinc
Crystal structure
Disulfides
Peptide Hydrolases
atrolysin C
Crotalus
Atoms
Snake Venoms
Molecules
Water
R Factors
Metalloproteases
Histidine
Methionine
H(2)-proteinase
Trimeresurus venoms
Ions
Calcium
Enzymes

Keywords

  • Crystal structure
  • Snake venom
  • Zinc-protease

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Crystal structure of H2-proteinase from the venom of Trimeresurus flavoviridis. / Kumasaka, Takashi; Yamamoto, Masaki; Moriyama, Hideaki; Tanaka, Nobuo; Sato, Mamoru; Katsube, Yukiteru; Yamakawa, Yoshio; Omori-Satoh, Tamotsu; Iwanaga, Sadaaki; Ueki, Tatzuo.

In: Journal of Biochemistry, Vol. 119, No. 1, 01.01.1996, p. 49-57.

Research output: Contribution to journalArticle

Kumasaka, T, Yamamoto, M, Moriyama, H, Tanaka, N, Sato, M, Katsube, Y, Yamakawa, Y, Omori-Satoh, T, Iwanaga, S & Ueki, T 1996, 'Crystal structure of H2-proteinase from the venom of Trimeresurus flavoviridis', Journal of Biochemistry, vol. 119, no. 1, pp. 49-57. https://doi.org/10.1093/oxfordjournals.jbchem.a021215
Kumasaka, Takashi ; Yamamoto, Masaki ; Moriyama, Hideaki ; Tanaka, Nobuo ; Sato, Mamoru ; Katsube, Yukiteru ; Yamakawa, Yoshio ; Omori-Satoh, Tamotsu ; Iwanaga, Sadaaki ; Ueki, Tatzuo. / Crystal structure of H2-proteinase from the venom of Trimeresurus flavoviridis. In: Journal of Biochemistry. 1996 ; Vol. 119, No. 1. pp. 49-57.
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