CRF07-BC strain dominates the HIV-1 epidemic in injection drug users in liangshan prefecture of Sichuan, China

Lin Li, Daying Wei, Wan Ling Hsu, Tianyi Li, Tao Gui, Charles Wood, Yongjian Liu, Hanping Li, Zuoyi Bao, Siyang Liu, Xiaolin Wang, Jingyun Li

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The Liangshan prefecture in Sichuan province is an area in China severely affected by the HIV epidemic, with intravenous drug use (IDU) as the main risk factor. No reports on HIV subtypes prevalent in IDUs in Liangshan prefecture could be found. In this study, we have characterized the genotypes of HIV-1 in the IDU population in Liangshan prefecture and further determined the phylogenetic relationship of the CRF07-BC strains to HIV-1 sequences from the other regions of China, including Xinjiang and Yunnan provinces, to explore the pattern and possible diffusion pathway of HIV-1 in these regions. HIV-1-seropositive drug-naive IDUs identified in Liangshan prefecture, Sichuan province were enrolled in 2009. Full-length gag and pol genes were amplified by reverse transcription and nested PCR and then sequenced. All of the sequences were subtyped. Phylogenetic trees were constructed using the neighbor-joining and maximum likelihood methods. Divergence times were estimated using a Bayesian molecular clock approach. CRF07-BC was found to be the predominant strain in IDUs in Liangshan prefecture (95.5%). The CRF07-BC strains from Liangshan prefecture were found to be intermixed with those from Yunnan province in phylogenetic trees. The CRF07-BC sequences from Xinjiang province can be grouped into several clusters, suggesting that the expansion of the CRF07-BC epidemic in Xinjiang province was the result of a local epidemic driven by multiple independent introductions in the late 1990s. Only low-level drug-resistant viruses were found in the IDU population. CRF07-BC strains from Liangshan prefecture were more similar to those from Yunnan province than those from Xinjiang province. This finding will contribute to our understanding of the distribution, the evolution, and the potential source of CRF07-BC founder strains, and will also provide useful information for the development of strategies to prevent transmission.

Original languageEnglish (US)
Pages (from-to)479-487
Number of pages9
JournalAIDS Research and Human Retroviruses
Volume31
Issue number5
DOIs
StatePublished - May 1 2015

Fingerprint

Drug Users
HIV-1
China
Injections
Pharmaceutical Preparations
HIV
pol Genes
gag Genes
Population
Reverse Transcription
Genotype
Viruses
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Infectious Diseases

Cite this

CRF07-BC strain dominates the HIV-1 epidemic in injection drug users in liangshan prefecture of Sichuan, China. / Li, Lin; Wei, Daying; Hsu, Wan Ling; Li, Tianyi; Gui, Tao; Wood, Charles; Liu, Yongjian; Li, Hanping; Bao, Zuoyi; Liu, Siyang; Wang, Xiaolin; Li, Jingyun.

In: AIDS Research and Human Retroviruses, Vol. 31, No. 5, 01.05.2015, p. 479-487.

Research output: Contribution to journalArticle

Li, Lin ; Wei, Daying ; Hsu, Wan Ling ; Li, Tianyi ; Gui, Tao ; Wood, Charles ; Liu, Yongjian ; Li, Hanping ; Bao, Zuoyi ; Liu, Siyang ; Wang, Xiaolin ; Li, Jingyun. / CRF07-BC strain dominates the HIV-1 epidemic in injection drug users in liangshan prefecture of Sichuan, China. In: AIDS Research and Human Retroviruses. 2015 ; Vol. 31, No. 5. pp. 479-487.
@article{fadb4ba4b0754d0ea579f980668110e8,
title = "CRF07-BC strain dominates the HIV-1 epidemic in injection drug users in liangshan prefecture of Sichuan, China",
abstract = "The Liangshan prefecture in Sichuan province is an area in China severely affected by the HIV epidemic, with intravenous drug use (IDU) as the main risk factor. No reports on HIV subtypes prevalent in IDUs in Liangshan prefecture could be found. In this study, we have characterized the genotypes of HIV-1 in the IDU population in Liangshan prefecture and further determined the phylogenetic relationship of the CRF07-BC strains to HIV-1 sequences from the other regions of China, including Xinjiang and Yunnan provinces, to explore the pattern and possible diffusion pathway of HIV-1 in these regions. HIV-1-seropositive drug-naive IDUs identified in Liangshan prefecture, Sichuan province were enrolled in 2009. Full-length gag and pol genes were amplified by reverse transcription and nested PCR and then sequenced. All of the sequences were subtyped. Phylogenetic trees were constructed using the neighbor-joining and maximum likelihood methods. Divergence times were estimated using a Bayesian molecular clock approach. CRF07-BC was found to be the predominant strain in IDUs in Liangshan prefecture (95.5{\%}). The CRF07-BC strains from Liangshan prefecture were found to be intermixed with those from Yunnan province in phylogenetic trees. The CRF07-BC sequences from Xinjiang province can be grouped into several clusters, suggesting that the expansion of the CRF07-BC epidemic in Xinjiang province was the result of a local epidemic driven by multiple independent introductions in the late 1990s. Only low-level drug-resistant viruses were found in the IDU population. CRF07-BC strains from Liangshan prefecture were more similar to those from Yunnan province than those from Xinjiang province. This finding will contribute to our understanding of the distribution, the evolution, and the potential source of CRF07-BC founder strains, and will also provide useful information for the development of strategies to prevent transmission.",
author = "Lin Li and Daying Wei and Hsu, {Wan Ling} and Tianyi Li and Tao Gui and Charles Wood and Yongjian Liu and Hanping Li and Zuoyi Bao and Siyang Liu and Xiaolin Wang and Jingyun Li",
year = "2015",
month = "5",
day = "1",
doi = "10.1089/aid.2014.0120",
language = "English (US)",
volume = "31",
pages = "479--487",
journal = "AIDS Research and Human Retroviruses",
issn = "0889-2229",
publisher = "Mary Ann Liebert Inc.",
number = "5",

}

TY - JOUR

T1 - CRF07-BC strain dominates the HIV-1 epidemic in injection drug users in liangshan prefecture of Sichuan, China

AU - Li, Lin

AU - Wei, Daying

AU - Hsu, Wan Ling

AU - Li, Tianyi

AU - Gui, Tao

AU - Wood, Charles

AU - Liu, Yongjian

AU - Li, Hanping

AU - Bao, Zuoyi

AU - Liu, Siyang

AU - Wang, Xiaolin

AU - Li, Jingyun

PY - 2015/5/1

Y1 - 2015/5/1

N2 - The Liangshan prefecture in Sichuan province is an area in China severely affected by the HIV epidemic, with intravenous drug use (IDU) as the main risk factor. No reports on HIV subtypes prevalent in IDUs in Liangshan prefecture could be found. In this study, we have characterized the genotypes of HIV-1 in the IDU population in Liangshan prefecture and further determined the phylogenetic relationship of the CRF07-BC strains to HIV-1 sequences from the other regions of China, including Xinjiang and Yunnan provinces, to explore the pattern and possible diffusion pathway of HIV-1 in these regions. HIV-1-seropositive drug-naive IDUs identified in Liangshan prefecture, Sichuan province were enrolled in 2009. Full-length gag and pol genes were amplified by reverse transcription and nested PCR and then sequenced. All of the sequences were subtyped. Phylogenetic trees were constructed using the neighbor-joining and maximum likelihood methods. Divergence times were estimated using a Bayesian molecular clock approach. CRF07-BC was found to be the predominant strain in IDUs in Liangshan prefecture (95.5%). The CRF07-BC strains from Liangshan prefecture were found to be intermixed with those from Yunnan province in phylogenetic trees. The CRF07-BC sequences from Xinjiang province can be grouped into several clusters, suggesting that the expansion of the CRF07-BC epidemic in Xinjiang province was the result of a local epidemic driven by multiple independent introductions in the late 1990s. Only low-level drug-resistant viruses were found in the IDU population. CRF07-BC strains from Liangshan prefecture were more similar to those from Yunnan province than those from Xinjiang province. This finding will contribute to our understanding of the distribution, the evolution, and the potential source of CRF07-BC founder strains, and will also provide useful information for the development of strategies to prevent transmission.

AB - The Liangshan prefecture in Sichuan province is an area in China severely affected by the HIV epidemic, with intravenous drug use (IDU) as the main risk factor. No reports on HIV subtypes prevalent in IDUs in Liangshan prefecture could be found. In this study, we have characterized the genotypes of HIV-1 in the IDU population in Liangshan prefecture and further determined the phylogenetic relationship of the CRF07-BC strains to HIV-1 sequences from the other regions of China, including Xinjiang and Yunnan provinces, to explore the pattern and possible diffusion pathway of HIV-1 in these regions. HIV-1-seropositive drug-naive IDUs identified in Liangshan prefecture, Sichuan province were enrolled in 2009. Full-length gag and pol genes were amplified by reverse transcription and nested PCR and then sequenced. All of the sequences were subtyped. Phylogenetic trees were constructed using the neighbor-joining and maximum likelihood methods. Divergence times were estimated using a Bayesian molecular clock approach. CRF07-BC was found to be the predominant strain in IDUs in Liangshan prefecture (95.5%). The CRF07-BC strains from Liangshan prefecture were found to be intermixed with those from Yunnan province in phylogenetic trees. The CRF07-BC sequences from Xinjiang province can be grouped into several clusters, suggesting that the expansion of the CRF07-BC epidemic in Xinjiang province was the result of a local epidemic driven by multiple independent introductions in the late 1990s. Only low-level drug-resistant viruses were found in the IDU population. CRF07-BC strains from Liangshan prefecture were more similar to those from Yunnan province than those from Xinjiang province. This finding will contribute to our understanding of the distribution, the evolution, and the potential source of CRF07-BC founder strains, and will also provide useful information for the development of strategies to prevent transmission.

UR - http://www.scopus.com/inward/record.url?scp=84928947189&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84928947189&partnerID=8YFLogxK

U2 - 10.1089/aid.2014.0120

DO - 10.1089/aid.2014.0120

M3 - Article

C2 - 25560398

AN - SCOPUS:84928947189

VL - 31

SP - 479

EP - 487

JO - AIDS Research and Human Retroviruses

JF - AIDS Research and Human Retroviruses

SN - 0889-2229

IS - 5

ER -