Coxsackievirus-induced chronic myocarditis in murine models

C. J. Gauntt, S. M. Tracy, N. Chapman, H. J. Wood, P. C. Kolbeck, A. G. Karaganis, C. L. Winfrey, M. W. Cunningham

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Challenge of several murine strains with two highly myocarditic variants of coxsackievirus B3 (CVB3) induced acute and chronic myocarditis, detectable at 21 and 45 days post-inoculation (p.i.). In-situ hybridization of coronal heart sections showing chronic inflammation with a radiolabelled CVB3 probe detected viral genomic RNA at day 7 p.i., but rarely at 21 or 45 days p.i., suggesting few murine heart cells actively replicate virus during chronic myocardial inflammation Data will be presented that favour an alternative hypothesis, i.e. autoimmune responses to shared epitopes among CVB3 proteins, cardiac myosin and myocardial cell surface proteins (molecular mimicry) can affect the severity of chronic inflammation. Mice inoculated with human cardiac myosin (HM) prior to a CVB3, challenge develop less myocarditis than mice inoculated with virus only, suggesting that antibodies stimulated by HM bind virus, reduce the virus burden and provide protection. Mice inoculated with HM only develop non-neutralizing antibodies against purified CVB3(m) particles. Several strains of mice inoculated with specific synthetic peptides of HM produce antibodies against CVB3(m) and/or develop cardiomyopathy. Thus antigen-challenged mice can produce antibodies which cross-react among CVB3(m) HM or cardiac cells to protect or exacerbate heart disease.

Original languageEnglish (US)
Pages (from-to)56-58
Number of pages3
JournalEuropean Heart Journal
Volume16
Issue numberSUPPL. O
StatePublished - Jan 1 1995

Fingerprint

Cardiac Myosins
Enterovirus
Myocarditis
Viruses
Antibodies
Inflammation
Molecular Mimicry
Viral RNA
Autoimmunity
Cardiomyopathies
In Situ Hybridization
Epitopes
Heart Diseases
Membrane Proteins
Antigens
Peptides

Keywords

  • Animal model
  • Autoimmune disease
  • Coxsackievirus
  • Enterovirus genomes
  • Mechanisms of pathogenicity
  • Myocarditis
  • Myosin epitopes
  • Shared epitopes

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Gauntt, C. J., Tracy, S. M., Chapman, N., Wood, H. J., Kolbeck, P. C., Karaganis, A. G., ... Cunningham, M. W. (1995). Coxsackievirus-induced chronic myocarditis in murine models. European Heart Journal, 16(SUPPL. O), 56-58.

Coxsackievirus-induced chronic myocarditis in murine models. / Gauntt, C. J.; Tracy, S. M.; Chapman, N.; Wood, H. J.; Kolbeck, P. C.; Karaganis, A. G.; Winfrey, C. L.; Cunningham, M. W.

In: European Heart Journal, Vol. 16, No. SUPPL. O, 01.01.1995, p. 56-58.

Research output: Contribution to journalArticle

Gauntt, CJ, Tracy, SM, Chapman, N, Wood, HJ, Kolbeck, PC, Karaganis, AG, Winfrey, CL & Cunningham, MW 1995, 'Coxsackievirus-induced chronic myocarditis in murine models', European Heart Journal, vol. 16, no. SUPPL. O, pp. 56-58.
Gauntt CJ, Tracy SM, Chapman N, Wood HJ, Kolbeck PC, Karaganis AG et al. Coxsackievirus-induced chronic myocarditis in murine models. European Heart Journal. 1995 Jan 1;16(SUPPL. O):56-58.
Gauntt, C. J. ; Tracy, S. M. ; Chapman, N. ; Wood, H. J. ; Kolbeck, P. C. ; Karaganis, A. G. ; Winfrey, C. L. ; Cunningham, M. W. / Coxsackievirus-induced chronic myocarditis in murine models. In: European Heart Journal. 1995 ; Vol. 16, No. SUPPL. O. pp. 56-58.
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