Covalent interactions of acetaldehyde with the actin/microfilament system

D. S. Xu, R. B. Jennett, S. L. Smith, Michael Floyd Sorrell, D. J. Tuma

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The covalent binding of [14C]acetaldehyde to purified rabbit skeletal muscle actin was characterized. As we have found for other cytoskeletal proteins, actin formed stable covalent adducts under reductive and non-reductive conditions. Under non-reductive conditions, individual and competition binding studies versus albumin both showed that the G-form of actin is more reactive toward acetaldehyde than the F-form. When proteins were compared on an 'equi-lysine' basis under non-reducing conditions, G-actin was found to preferentially complete with albumin for binding to acetaldehyde. Time-course dialysis studies indicated that acetaldehyde-actin adducts become more stable with prolonged incubation at 37°C. These data raise the possibility that actin could be a preferential target for adduct formationin cellular systems and will serve as the basis for ongoing studies aimed at defining the role of acetaldehyde-protein adducts in ethanol-induced cell injury.

Original languageEnglish (US)
Pages (from-to)281-289
Number of pages9
JournalAlcohol and Alcoholism
Volume24
Issue number4
DOIs
StatePublished - Jan 1 1989

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Acetaldehyde
Actin Cytoskeleton
Actins
Albumins
Cytoskeletal Proteins
Dialysis
Lysine
Muscle
Skeletal Muscle
Proteins
Ethanol
Rabbits
Wounds and Injuries

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Toxicology
  • Psychiatry and Mental health

Cite this

Covalent interactions of acetaldehyde with the actin/microfilament system. / Xu, D. S.; Jennett, R. B.; Smith, S. L.; Sorrell, Michael Floyd; Tuma, D. J.

In: Alcohol and Alcoholism, Vol. 24, No. 4, 01.01.1989, p. 281-289.

Research output: Contribution to journalArticle

Xu, D. S. ; Jennett, R. B. ; Smith, S. L. ; Sorrell, Michael Floyd ; Tuma, D. J. / Covalent interactions of acetaldehyde with the actin/microfilament system. In: Alcohol and Alcoholism. 1989 ; Vol. 24, No. 4. pp. 281-289.
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