Covalent binding of acetaldehyde to hepatic proteins during ethanol oxidation

Victor A. Medina, Terrence Donohue, Michael Floyd Sorrell, Dean J. Tuma

Research output: Contribution to journalArticle

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Abstract

Acetaldehyde production and the radiolabeling of hepatic proteins were determined in rat liver slices incubated with 14C-ethanol (10 mmol/L). Significant labeling of hepatic proteins occurred in the presence of protein synthesis inhibitors, indicating that, under these conditions, the radiolabeling of protein did not occur via de novo protein synthesis. Additional experiments indicated that the major source of protein-bound radioactivity derived from 14C-ethanol oxidation was the formation of 14C-acetaldehyde adducts with proteins. This conclusion was made from observations that pyrazole, an inhibitor of ethanol oxidation and, therefore, acetaldehyde formation, decreased radiolabeling of protein, whereas cyanamide, which elevated hepatic acetaldehyde levels, markedly increased the labeling of protein. Furthermore, l-cysteine, which can bind acetaldehyde and, therefore, act as an acetaldehyde trap, substantially reduced protein-bound radioactivity. It was also demonstrated that acetaldehyde formed both stable and unstable adducts with hepatic proteins and that unstable adducts may undergo conversion to form stable adducts during incubation.

Original languageEnglish (US)
Pages (from-to)5-10
Number of pages6
JournalThe Journal of Laboratory and Clinical Medicine
Volume105
Issue number1
StatePublished - Jan 1985

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Acetaldehyde
Ethanol
Oxidation
Liver
Proteins
Radioactivity
Labeling
Cyanamide
Protein Synthesis Inhibitors
Corrosion inhibitors
Cysteine
Rats

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Covalent binding of acetaldehyde to hepatic proteins during ethanol oxidation. / Medina, Victor A.; Donohue, Terrence; Sorrell, Michael Floyd; Tuma, Dean J.

In: The Journal of Laboratory and Clinical Medicine, Vol. 105, No. 1, 01.1985, p. 5-10.

Research output: Contribution to journalArticle

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