Correlation of mucosal disaccharidase activities with histology in evaluation of rejection following intestinal transplantation

Stuart S. Kaufman, James Lowell Wisecarver, Elizabeth I. Ruby, Ira J. Fox, Debra L. Sudan, Todd J. Pillen, Anil Dhawan, Simon P. Horslen, Jon A. Vanderhoof, Stanley J Radio, Rodney Smith Markin, Alan Norman Langnas

Research output: Contribution to journalArticle

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Abstract

Following intestinal transplantation, we have found that recovery from severe rejection may be difficult to identify. In this study we sought to ascertain whether concurrent determination of mucosal disaccharidase activities and histologic assessment improves the accuracy of diagnosis of rejection. Histologic changes were graded blindly using a standard set of diagnostic criteria, and these changes were compared over time to maltase, sucrase, lactase, and palatinase activities in four pediatric patients under treatment for severe rejection. The histologic criteria, which included magnitude of enterocyte loss, degree of granulation tissue, severity of villus atrophy, and frequency of apoptosis and cryptitis, were found to correlate with one another over time irrespective of outcome (r = 0.72 to r= 0.85). Enzyme activities were also correlated with each other over time (r = 0.64 to r= 0.80). However, the correlation between histologic diagnosis and enzyme activity was weaker (r = -0.48 to r = -0.57). Furthermore, neither histologic nor enzyme evaluation early in the course of rejection predicted ultimate clinical outcome. The results of this investigation show that determination of mucosal disaccharidase activity provides no additional useful information concerning efficacy of anti-rejection therapy as compared to histologic analysis alone.

Original languageEnglish (US)
Pages (from-to)134-138
Number of pages5
JournalPediatric Transplantation
Volume2
Issue number2
StatePublished - Dec 1 1998

Fingerprint

Disaccharidases
Histology
Transplantation
Enzymes
Sucrase
Lactase
alpha-Glucosidases
Granulation Tissue
Enterocytes
Immunosuppression
Atrophy
Pediatrics
Apoptosis
Therapeutics

Keywords

  • Allograft
  • Disaccharidase
  • Lactase
  • Maltase
  • Pathology
  • Rejection
  • Small intestine
  • Sucrase

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Transplantation

Cite this

Correlation of mucosal disaccharidase activities with histology in evaluation of rejection following intestinal transplantation. / Kaufman, Stuart S.; Wisecarver, James Lowell; Ruby, Elizabeth I.; Fox, Ira J.; Sudan, Debra L.; Pillen, Todd J.; Dhawan, Anil; Horslen, Simon P.; Vanderhoof, Jon A.; Radio, Stanley J; Markin, Rodney Smith; Langnas, Alan Norman.

In: Pediatric Transplantation, Vol. 2, No. 2, 01.12.1998, p. 134-138.

Research output: Contribution to journalArticle

Kaufman, Stuart S. ; Wisecarver, James Lowell ; Ruby, Elizabeth I. ; Fox, Ira J. ; Sudan, Debra L. ; Pillen, Todd J. ; Dhawan, Anil ; Horslen, Simon P. ; Vanderhoof, Jon A. ; Radio, Stanley J ; Markin, Rodney Smith ; Langnas, Alan Norman. / Correlation of mucosal disaccharidase activities with histology in evaluation of rejection following intestinal transplantation. In: Pediatric Transplantation. 1998 ; Vol. 2, No. 2. pp. 134-138.
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