Correlation of enterohemorrhagic Escherichia coli 0157 prevalence in feces, hides, and carcasses of beef cattle during processing

Robert O. Elder, James E. Keen, Gregory R. Siragusa, Genevieve A. Barkocy-Gallagher, Mohammad Koohmaraie, William W. Laegreid

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Abstract

A survey was performed to estimate the frequency of enterohemorrhagic Escherichia coli O157:H7 or O157:nonmotile (EHEC O157) in feces and on hides within groups of fed cattle from single sources (lots) presented for slaughter at meat processing plants in the Midwestern United States, as well as frequency of carcass contamination during processing from cattle within the same lots. Of 29 lots sampled, 72% had at least one EHEC O157-positive fecal sample and 38% had positive hide samples. Overall, EHEC O157 prevalence in feces and on hides was 28% (91 of 327) and 11% (38 of 355), respectively. Carcass samples were taken at three points during processing: preevisceration, postevisceration before antimicrobial intervention, and postprocessing after carcasses entered the cooler. Of 30 lots sampled, 87% had at least one EHEC O157-positive preevisceration sample, 57% of lots were positive postevisceration, and 17% had positive postprocessing samples. Prevalence of EHEC O157 in the three postprocessing samples was 43% (148 of 341), 18% (59 of 332) and 2% (6 of 330), respectively. Reduction in carcass prevalence from preevisceration to postprocessing suggests that sanitary procedures were effective within the processing plants. Fecal and hide prevalence were significantly correlated with carcass contamination (P = 0.001), indicating a role for control of EHEC O157 in live cattle.

Original languageEnglish (US)
Pages (from-to)2999-3003
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume97
Issue number7
DOIs
StatePublished - Mar 28 2000

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Enterohemorrhagic Escherichia coli
Escherichia coli O157
Feces
Midwestern United States
Meat

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Correlation of enterohemorrhagic Escherichia coli 0157 prevalence in feces, hides, and carcasses of beef cattle during processing. / Elder, Robert O.; Keen, James E.; Siragusa, Gregory R.; Barkocy-Gallagher, Genevieve A.; Koohmaraie, Mohammad; Laegreid, William W.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, No. 7, 28.03.2000, p. 2999-3003.

Research output: Contribution to journalArticle

Elder, Robert O. ; Keen, James E. ; Siragusa, Gregory R. ; Barkocy-Gallagher, Genevieve A. ; Koohmaraie, Mohammad ; Laegreid, William W. / Correlation of enterohemorrhagic Escherichia coli 0157 prevalence in feces, hides, and carcasses of beef cattle during processing. In: Proceedings of the National Academy of Sciences of the United States of America. 2000 ; Vol. 97, No. 7. pp. 2999-3003.
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