Correlation of acetate catabolism and growth yield in Staphylococcus aureus

Implications for host-pathogen interactions

Greg A Somerville, Battouli Saïd-Salim, Jaala M. Wickman, Sandra J. Raffel, Barry N. Kreiswirth, James M. Musser

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Recently, we reported that the prototypical Staphylococcus aureus strain RN6390 (a derivative of NCTC 8325) had significantly reduced aconitase activity relative to a diverse group of S. aureus isolates, leading to the hypothesis that strain RN6390 has impaired tricarboxylic acid (TCA) cycle-mediated acetate catabolism. Analysis of the culture supernatant from RN6390 confirmed that acetate was incompletely catabolized, suggesting that the ability to catabolize acetate can be lost by S. aureus. To test this hypothesis, we examined the carbon catabolism of the S. aureus strains whose genome sequences are publicly available. All strains catabolized glucose and excreted acetate into the culture medium. However, strains NCTC 8325 and N315 failed to catabolize acetate during the postexponential growth phase, resulting in significantly lower growth yields relative to strains that catabolized acetate. Strains NCTC 8325 and RN6390 contained an 11-bp deletion in rsbU, the gene encoding a positive regulator of the alternative sigma factor σB encoded by sigB. An isogenic derivative strain of RN6390 containing the wild-type rsbU gene had significantly increased acetate catabolism, demonstrating that σB is required for acetate catabolism. Taken together, the data suggest that naturally occurring mutations can alter the ability of S. aureus to catabolize acetate, a surprising discovery, as TCA cycle function has been demonstrated to be involved in the virulence, survival, and persistence of several pathogenic organisms. Additionally, these mutations decrease the fitness of S. aureus by reducing the number of progeny placed into subsequent generations, suggesting that in certain situations a decreased growth yield is advantageous.

Original languageEnglish (US)
Pages (from-to)4724-4732
Number of pages9
JournalInfection and immunity
Volume71
Issue number8
DOIs
StatePublished - Aug 1 2003

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Host-Pathogen Interactions
Staphylococcus aureus
Acetates
Growth
Citric Acid Cycle
Aconitate Hydratase
Sigma Factor
Mutation
Genes
Virulence
Culture Media
Carbon
Genome
Glucose

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Correlation of acetate catabolism and growth yield in Staphylococcus aureus : Implications for host-pathogen interactions. / Somerville, Greg A; Saïd-Salim, Battouli; Wickman, Jaala M.; Raffel, Sandra J.; Kreiswirth, Barry N.; Musser, James M.

In: Infection and immunity, Vol. 71, No. 8, 01.08.2003, p. 4724-4732.

Research output: Contribution to journalArticle

Somerville, Greg A ; Saïd-Salim, Battouli ; Wickman, Jaala M. ; Raffel, Sandra J. ; Kreiswirth, Barry N. ; Musser, James M. / Correlation of acetate catabolism and growth yield in Staphylococcus aureus : Implications for host-pathogen interactions. In: Infection and immunity. 2003 ; Vol. 71, No. 8. pp. 4724-4732.
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