Correlation between histone lysine methylation and developmental changes at the chicken β-globin locus

M. D. Litt, M. Simpson, M. Gaszner, C. D. Allis, G. Felsenfeld

Research output: Contribution to journalArticle

498 Citations (Scopus)

Abstract

Methylation of histones at specific residues plays an important role in transcriptional regulation. Chromatin immunoprecipitation of dimethylated lysine 9 on histone H3 across 53 kilobases of the chicken β-globin locus during erythropoiesis shows an almost complete anticorrelation between regions of elevated lysine 9 methylation and acetylation. Lysine 9 is methylated most over constitutive condensed chromatin and developmentally inactive globin genes. In contrast, lysine 4 methylation of histone H3 correlates with H3 acetylation. These results lead us to propose a mechanism by which the insulator in the β-globin locus can protect the globin genes from being silenced by adjacent condensed chromatin.

Original languageEnglish (US)
Pages (from-to)2453-2455
Number of pages3
JournalScience
Volume293
Issue number5539
DOIs
StatePublished - Sep 28 2001

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Globins
Histones
Methylation
Lysine
Chickens
Acetylation
Chromatin
Chromatin Immunoprecipitation
Erythropoiesis
Genes

ASJC Scopus subject areas

  • General

Cite this

Litt, M. D., Simpson, M., Gaszner, M., Allis, C. D., & Felsenfeld, G. (2001). Correlation between histone lysine methylation and developmental changes at the chicken β-globin locus. Science, 293(5539), 2453-2455. https://doi.org/10.1126/science.1064413

Correlation between histone lysine methylation and developmental changes at the chicken β-globin locus. / Litt, M. D.; Simpson, M.; Gaszner, M.; Allis, C. D.; Felsenfeld, G.

In: Science, Vol. 293, No. 5539, 28.09.2001, p. 2453-2455.

Research output: Contribution to journalArticle

Litt, MD, Simpson, M, Gaszner, M, Allis, CD & Felsenfeld, G 2001, 'Correlation between histone lysine methylation and developmental changes at the chicken β-globin locus', Science, vol. 293, no. 5539, pp. 2453-2455. https://doi.org/10.1126/science.1064413
Litt, M. D. ; Simpson, M. ; Gaszner, M. ; Allis, C. D. ; Felsenfeld, G. / Correlation between histone lysine methylation and developmental changes at the chicken β-globin locus. In: Science. 2001 ; Vol. 293, No. 5539. pp. 2453-2455.
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