Correct identification of genes from serial analysis of gene expression tag sequences

Sanggyu Lee, Terry Clark, Jianjun Chen, Guolin Zhou, L. Ridgway Scott, Janet D. Rowley, San Ming Wang

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

SAGE (serial analysis of gene expression) is a remarkable technique for genome-wide analysis of gene expression. It is crucial to understand the extent to which SAGE can accurately indicate a gene or expressed sequence tag (EST) with a single tag. We analyzed the effect of the size of SAGE tag on gene identification. Our observation indicates that SAGE tags are in general not long enough to achieve the degree of uniqueness of identification originally envisaged. Our observations also indicate that the limitation of using SAGE tag to identify a gene can be overcome by converting SAGE tags into longer 3′ EST sequences with the generation of longer cDNA fragments from SAGE tages for gene identification (GLGI) method.

Original languageEnglish (US)
Pages (from-to)598-602
Number of pages5
JournalGenomics
Volume79
Issue number4
DOIs
Publication statusPublished - Jan 1 2002

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Keywords

  • GLGI
  • Gene expression
  • Gene identification
  • SAGE

ASJC Scopus subject areas

  • Genetics

Cite this

Lee, S., Clark, T., Chen, J., Zhou, G., Scott, L. R., Rowley, J. D., & Wang, S. M. (2002). Correct identification of genes from serial analysis of gene expression tag sequences. Genomics, 79(4), 598-602. https://doi.org/10.1006/geno.2002.6730