Copper tolerance in fathead minnows

I. The role of genetic and nongenetic factors

Alan S Kolok, Elizabeth B. Peake, Laura L. Tierney, Shaun A. Roark, Robert B. Noble, Kyoungah See, Sheldon I. Guttman

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Swim performances of male and female fathead minnows (Pimephales promelas) from three different suppliers were determined before and after an 8- to 9-d exposure to 175 μg/L copper (Cu). The reduction in swim performance (delta) due to the Cu exposure varied widely among individual fish, but was surprisingly consistent from one supplier to the next and between males and females. Genetic analysis of the individuals revealed significant correlations between delta and genotypic variation at the glucosephosphate isomerase-1, phosphoglucomutase-1, and lactate dehydrogenase-2 enzyme loci. Based upon delta, the most Cu-resistant fathead minnows were bred together, as were the most Cu-susceptible individuals and two groups of unselected minnows. Larvae produced by each group of adults were subjected to a survival test. The median lethal concentration (LC50) for larvae produced by Cu-resistant adults was significantly greater than the LC50s for the control groups. Surprisingly, the LC50 for the larvae produced by Cu-susceptible adults was also significantly greater than the LC50s for the control groups, but not significantly different from the larvae produced by Cu-resistant parents. While Cu tolerance has a genetic component in fathead minnows, the Cu tolerance of larval fish appears to be influenced by nongenetic as well as genetic factors.

Original languageEnglish (US)
Pages (from-to)200-207
Number of pages8
JournalEnvironmental Toxicology and Chemistry
Volume23
Issue number1
DOIs
StatePublished - Jan 1 2004

Fingerprint

Cyprinidae
Fish
Larva
Copper
tolerance
Phosphoglucomutase
copper
Glucose-6-Phosphate Isomerase
larva
Fishes
Control Groups
genetic analysis
Enzymes
fish
enzyme
lactate dehydrogenase 2
exposure

Keywords

  • Allozymic variation
  • Copper
  • Critical swim speed
  • Median lethal concentration
  • Sublethal exposure

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Kolok, A. S., Peake, E. B., Tierney, L. L., Roark, S. A., Noble, R. B., See, K., & Guttman, S. I. (2004). Copper tolerance in fathead minnows: I. The role of genetic and nongenetic factors. Environmental Toxicology and Chemistry, 23(1), 200-207. https://doi.org/10.1897/02-609

Copper tolerance in fathead minnows : I. The role of genetic and nongenetic factors. / Kolok, Alan S; Peake, Elizabeth B.; Tierney, Laura L.; Roark, Shaun A.; Noble, Robert B.; See, Kyoungah; Guttman, Sheldon I.

In: Environmental Toxicology and Chemistry, Vol. 23, No. 1, 01.01.2004, p. 200-207.

Research output: Contribution to journalArticle

Kolok, AS, Peake, EB, Tierney, LL, Roark, SA, Noble, RB, See, K & Guttman, SI 2004, 'Copper tolerance in fathead minnows: I. The role of genetic and nongenetic factors', Environmental Toxicology and Chemistry, vol. 23, no. 1, pp. 200-207. https://doi.org/10.1897/02-609
Kolok, Alan S ; Peake, Elizabeth B. ; Tierney, Laura L. ; Roark, Shaun A. ; Noble, Robert B. ; See, Kyoungah ; Guttman, Sheldon I. / Copper tolerance in fathead minnows : I. The role of genetic and nongenetic factors. In: Environmental Toxicology and Chemistry. 2004 ; Vol. 23, No. 1. pp. 200-207.
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