Coordinated and distinct functions of velvet proteins in Fusarium verticillioides

Nan Lan, Hanxing Zhang, Chengcheng Hu, Wenzhao Wang, Ana M. Calvo, Steven D Harris, She Chen, Shaojie Li

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Velvet-domain-containing proteins are broadly distributed within the fungal kingdom. In the corn pathogen Fusarium verticillioides, previous studies showed that the velvet protein F. verticillioides VE1 (FvVE1) is critical for morphological development, colony hydrophobicity, toxin production, and pathogenicity. In this study, tandem affinity purification of FvVE1 revealed that FvVE1 can form a complex with the velvet proteins F. verticillioides VelB (FvVelB) and FvVelC. Phenotypic characterization of gene knockout mutants showed that, as in the case of FvVE1, FvVelB regulated conidial size, hyphal hydrophobicity, fumonisin production, and oxidant resistance, while FvVelC was dispensable for these biological processes. Comparative transcriptional analysis of eight genes involved in the ROS (reactive oxygen species) removal system revealed that both FvVE1 and FvVelB positively regulated the transcription of a catalase-encoding gene, F. verticillioides CAT2 (FvCAT2). Deletion of FvCAT2 resulted in reduced oxidant resistance, providing further explanation of the regulation of oxidant resistance by velvet proteins in the fungal kingdom.

Original languageEnglish (US)
Pages (from-to)909-918
Number of pages10
JournalEukaryotic Cell
Volume13
Issue number7
DOIs
StatePublished - Jan 1 2014

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Fusarium
Oxidants
Hydrophobic and Hydrophilic Interactions
Fumonisins
Biological Phenomena
Gene Knockout Techniques
Proteins
Fungal Proteins
Catalase
Genes
Zea mays
Virulence
Reactive Oxygen Species

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Lan, N., Zhang, H., Hu, C., Wang, W., Calvo, A. M., Harris, S. D., ... Li, S. (2014). Coordinated and distinct functions of velvet proteins in Fusarium verticillioides. Eukaryotic Cell, 13(7), 909-918. https://doi.org/10.1128/EC.00022-14

Coordinated and distinct functions of velvet proteins in Fusarium verticillioides. / Lan, Nan; Zhang, Hanxing; Hu, Chengcheng; Wang, Wenzhao; Calvo, Ana M.; Harris, Steven D; Chen, She; Li, Shaojie.

In: Eukaryotic Cell, Vol. 13, No. 7, 01.01.2014, p. 909-918.

Research output: Contribution to journalArticle

Lan, N, Zhang, H, Hu, C, Wang, W, Calvo, AM, Harris, SD, Chen, S & Li, S 2014, 'Coordinated and distinct functions of velvet proteins in Fusarium verticillioides', Eukaryotic Cell, vol. 13, no. 7, pp. 909-918. https://doi.org/10.1128/EC.00022-14
Lan, Nan ; Zhang, Hanxing ; Hu, Chengcheng ; Wang, Wenzhao ; Calvo, Ana M. ; Harris, Steven D ; Chen, She ; Li, Shaojie. / Coordinated and distinct functions of velvet proteins in Fusarium verticillioides. In: Eukaryotic Cell. 2014 ; Vol. 13, No. 7. pp. 909-918.
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