Coordinate induction of alcohol dehydrogenase 1, aldolase, and other anaerobic RNAs in maize

S. Hake, P. M. Kelley, W. C. Taylor, M. Freeling

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Abstract

Anaerobiosis results in the selective synthesis of a particular set of polypeptides in the maize root including the two alcohol dehydrogenases (Sachs, M.M., Freeling, M., and Okimoto, R. (1980) Cell 20, 761-768), pyruvate decarboxylase (Wignarajah, K., and Greenway, H. (1976) New Phytol. 77, 575-584; Laszlo, A., and St. Lawrence, P. (1983) Mol. Gen. Genet. 192, 110-117), glucose phosphate isomerase (Kelley, P.M., and Freeling, M. (1984) J. Biol. Chem. 259, 673-677) and aldolase (Kelley, P.M., and Freeling, M. (1984) J. Biol. Chem. 259, 14180- 14183). This report describes the identification and characterization of cDNA clones to five different mRNA species induced upon anaerobic shock. Immunoprecipitation of hybrid- selected translation polypeptides has determined the identity of the cDNA clone for fructose-1,6-diphosphate aldolase mRNA. Quantitative hybridization analysis of anaerobic mRNAs using the cDNA clones has shown that there is not a simultaneous accumulation anaerobic mRNAs. Upon reintroduction of air, the anaerobic mRNAs disappear rapidly and at approximately the same rate. A translocation line that generates progeny that contain 1, 2, and 3 doses of the long arm of chromosome one (1L) allowed us to test for clustering of the anaerobic genes; two of the anaerobic genes tested do not reside with Adh1 and Phi1 on the long arm of chromosome 1.

Original languageEnglish (US)
Pages (from-to)5050-5054
Number of pages5
JournalJournal of Biological Chemistry
Volume260
Issue number8
Publication statusPublished - Jan 1 1985

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Hake, S., Kelley, P. M., Taylor, W. C., & Freeling, M. (1985). Coordinate induction of alcohol dehydrogenase 1, aldolase, and other anaerobic RNAs in maize. Journal of Biological Chemistry, 260(8), 5050-5054.