Coordinate induction of alcohol dehydrogenase 1, aldolase, and other anaerobic RNAs in maize

S. Hake, P. M. Kelley, W. C. Taylor, M. Freeling

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Anaerobiosis results in the selective synthesis of a particular set of polypeptides in the maize root including the two alcohol dehydrogenases (Sachs, M.M., Freeling, M., and Okimoto, R. (1980) Cell 20, 761-768), pyruvate decarboxylase (Wignarajah, K., and Greenway, H. (1976) New Phytol. 77, 575-584; Laszlo, A., and St. Lawrence, P. (1983) Mol. Gen. Genet. 192, 110-117), glucose phosphate isomerase (Kelley, P.M., and Freeling, M. (1984) J. Biol. Chem. 259, 673-677) and aldolase (Kelley, P.M., and Freeling, M. (1984) J. Biol. Chem. 259, 14180- 14183). This report describes the identification and characterization of cDNA clones to five different mRNA species induced upon anaerobic shock. Immunoprecipitation of hybrid- selected translation polypeptides has determined the identity of the cDNA clone for fructose-1,6-diphosphate aldolase mRNA. Quantitative hybridization analysis of anaerobic mRNAs using the cDNA clones has shown that there is not a simultaneous accumulation anaerobic mRNAs. Upon reintroduction of air, the anaerobic mRNAs disappear rapidly and at approximately the same rate. A translocation line that generates progeny that contain 1, 2, and 3 doses of the long arm of chromosome one (1L) allowed us to test for clustering of the anaerobic genes; two of the anaerobic genes tested do not reside with Adh1 and Phi1 on the long arm of chromosome 1.

Original languageEnglish (US)
Pages (from-to)5050-5054
Number of pages5
JournalJournal of Biological Chemistry
Volume260
Issue number8
StatePublished - Jan 1 1985

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Fructose-Bisphosphate Aldolase
Alcohol Dehydrogenase
Zea mays
RNA
Messenger RNA
Complementary DNA
Clone Cells
Chromosomes
xylose isomerase
Genes
Phytol
Pyruvate Decarboxylase
Anaerobiosis
Viverridae
Isomerases
Peptides
Chromosomes, Human, Pair 1
Immunoprecipitation
Cluster Analysis
Shock

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Hake, S., Kelley, P. M., Taylor, W. C., & Freeling, M. (1985). Coordinate induction of alcohol dehydrogenase 1, aldolase, and other anaerobic RNAs in maize. Journal of Biological Chemistry, 260(8), 5050-5054.

Coordinate induction of alcohol dehydrogenase 1, aldolase, and other anaerobic RNAs in maize. / Hake, S.; Kelley, P. M.; Taylor, W. C.; Freeling, M.

In: Journal of Biological Chemistry, Vol. 260, No. 8, 01.01.1985, p. 5050-5054.

Research output: Contribution to journalArticle

Hake, S, Kelley, PM, Taylor, WC & Freeling, M 1985, 'Coordinate induction of alcohol dehydrogenase 1, aldolase, and other anaerobic RNAs in maize', Journal of Biological Chemistry, vol. 260, no. 8, pp. 5050-5054.
Hake, S. ; Kelley, P. M. ; Taylor, W. C. ; Freeling, M. / Coordinate induction of alcohol dehydrogenase 1, aldolase, and other anaerobic RNAs in maize. In: Journal of Biological Chemistry. 1985 ; Vol. 260, No. 8. pp. 5050-5054.
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