Cooperative processing of primary miRNAs by DUS16 and DCL3 in the unicellular green alga Chlamydomonas reinhardtii

Tomohito Yamasaki, Heriberto Cerutti

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We have previously reported that the RNA-binding protein Dull slicer 16 (DUS16) plays a key role in the processing of primary miRNAs (pri-miRNAs) in the unicellular green alga Chlamydomonas reinhardtii. In the present report, we elaborate on the interaction of DUS16 with Dicer-like 3 (DCL3) during pri-miRNA processing. Comprehensive analyses of small RNA libraries derived from mutant and wild-type algal strains allowed the de novo prediction of 35 pri-miRNA genes, including 9 previously unknown ones. The pri-miRNAs dependent on DUS16 for processing largely overlapped with those dependent on DCL3. Our findings suggest that DUS16 and DCL3 work cooperatively, presumably as components of a microprocessor complex, in the processing of the majority of pri-miRNAs in C. reinhardtii.

Original languageEnglish (US)
Article numbere1280208
JournalCommunicative and Integrative Biology
Issue number1
Publication statusPublished - Jan 2 2017



  • Argonaute
  • Chlamydomonas reinhardtii
  • Dicer
  • RNA-binding protein
  • miRNA
  • small RNA-seq

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

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