Convergence of non-clathrin- and clathrin-derived endosomes involves Arf6 inactivation and changes in phosphoinositides

Naava Naslavsky, Roberto Weigert, Julie G. Donaldson

Research output: Contribution to journalArticle

205 Citations (Scopus)

Abstract

The trafficking of two plasma membrane (PM) proteins that lack clathrin internalization sequences, major histocompatibility complex class I (MHCI), and interleukin 2 receptor α subunit (Tac) was compared with that of PM proteins internalized via clathrin. MHCI and Tac were internalized into endosomes that were distinct from those containing clathrin cargo. At later times, a fraction of these internalized membranes were observed in Arf6-associated, tubular recycling endosomes whereas another fraction acquired early endosomal autoantigen 1 (EEA1) before fusion with the "classical" early endosomes containing the clathrin-dependent cargo, LDL. After convergence, cargo molecules from both pathways eventually arrived, in a Rab7-dependent manner, at late endosomes and were degraded. Expression of a constitutively active mutant of Arf6, Q67L, caused MHCI and Tac to accumulate in enlarged PIP2-enriched vacuoles, devoid of EEA1 and inhibited their fusion with clathrin cargo-containing endosomes and hence blocked degradation. By contrast, trafficking and degradation of clathrin-cargo was not affected. A similar block in transport of MHCI and Tac was reversibly induced by a PI3-kinase inhibitor, implying that inactivation of Arf6 and acquisition of PI3P are required for convergence of endosomes arising from these two pathways.

Original languageEnglish (US)
Pages (from-to)417-431
Number of pages15
JournalMolecular biology of the cell
Volume14
Issue number2
DOIs
StatePublished - Feb 1 2003

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Clathrin
Endosomes
Phosphatidylinositols
Major Histocompatibility Complex
Autoantigens
Blood Proteins
Membrane Proteins
Cell Membrane
Interleukin-2 Receptors
Vacuoles
Phosphatidylinositol 3-Kinases
Membranes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Convergence of non-clathrin- and clathrin-derived endosomes involves Arf6 inactivation and changes in phosphoinositides. / Naslavsky, Naava; Weigert, Roberto; Donaldson, Julie G.

In: Molecular biology of the cell, Vol. 14, No. 2, 01.02.2003, p. 417-431.

Research output: Contribution to journalArticle

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