Controlled release systems for DNA delivery

Angela K. Pannier, Lonnie D. Shea

Research output: Contribution to journalReview article

170 Citations (Scopus)

Abstract

Adapting controlled release technologies to the delivery of DNA has the potential to overcome extracellular barriers that limit gene therapy. Controlled release systems can enhance gene delivery and increase the extent and duration of transgene expression relative to more traditional delivery methods (e.g., injection). These systems typically deliver vectors locally, which can avoid distribution to distant tissues, decrease toxicity to nontarget cells, and reduce the immune response to the vector. Delivery vehicles for controlled release are fabricated from natural and synthetic polymers, which function either by releasing the vector into the local tissue environment or by maintaining the vector at the polymer surface. Vector release or binding is regulated by the effective affinity of the vector for the polymer, which depends upon the strength of molecular interactions. These interactions occur through nonspecific binding based on vector and polymer composition or through the incorporation of complementary binding sites (e.g., biotin-avidin). This review examines the delivery of nonviral and viral vectors from natural and synthetic polymers and presents opportunities for continuing developments to increase their applicability.

Original languageEnglish (US)
Pages (from-to)19-26
Number of pages8
JournalMolecular Therapy
Volume10
Issue number1
DOIs
StatePublished - Jul 1 2004

Fingerprint

Polymers
DNA
Avidin
Biotin
Transgenes
Genetic Therapy
Binding Sites
Technology
Injections
Genes

Keywords

  • Biomaterials
  • Gene therapy
  • Plasmid
  • Substrate-mediated delivery
  • Tissue engineering

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Pharmacology
  • Drug Discovery

Cite this

Controlled release systems for DNA delivery. / Pannier, Angela K.; Shea, Lonnie D.

In: Molecular Therapy, Vol. 10, No. 1, 01.07.2004, p. 19-26.

Research output: Contribution to journalReview article

Pannier, Angela K. ; Shea, Lonnie D. / Controlled release systems for DNA delivery. In: Molecular Therapy. 2004 ; Vol. 10, No. 1. pp. 19-26.
@article{cc068e355fe044508a0a47e63464f14a,
title = "Controlled release systems for DNA delivery",
abstract = "Adapting controlled release technologies to the delivery of DNA has the potential to overcome extracellular barriers that limit gene therapy. Controlled release systems can enhance gene delivery and increase the extent and duration of transgene expression relative to more traditional delivery methods (e.g., injection). These systems typically deliver vectors locally, which can avoid distribution to distant tissues, decrease toxicity to nontarget cells, and reduce the immune response to the vector. Delivery vehicles for controlled release are fabricated from natural and synthetic polymers, which function either by releasing the vector into the local tissue environment or by maintaining the vector at the polymer surface. Vector release or binding is regulated by the effective affinity of the vector for the polymer, which depends upon the strength of molecular interactions. These interactions occur through nonspecific binding based on vector and polymer composition or through the incorporation of complementary binding sites (e.g., biotin-avidin). This review examines the delivery of nonviral and viral vectors from natural and synthetic polymers and presents opportunities for continuing developments to increase their applicability.",
keywords = "Biomaterials, Gene therapy, Plasmid, Substrate-mediated delivery, Tissue engineering",
author = "Pannier, {Angela K.} and Shea, {Lonnie D.}",
year = "2004",
month = "7",
day = "1",
doi = "10.1016/j.ymthe.2004.03.020",
language = "English (US)",
volume = "10",
pages = "19--26",
journal = "Molecular Therapy",
issn = "1525-0016",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Controlled release systems for DNA delivery

AU - Pannier, Angela K.

AU - Shea, Lonnie D.

PY - 2004/7/1

Y1 - 2004/7/1

N2 - Adapting controlled release technologies to the delivery of DNA has the potential to overcome extracellular barriers that limit gene therapy. Controlled release systems can enhance gene delivery and increase the extent and duration of transgene expression relative to more traditional delivery methods (e.g., injection). These systems typically deliver vectors locally, which can avoid distribution to distant tissues, decrease toxicity to nontarget cells, and reduce the immune response to the vector. Delivery vehicles for controlled release are fabricated from natural and synthetic polymers, which function either by releasing the vector into the local tissue environment or by maintaining the vector at the polymer surface. Vector release or binding is regulated by the effective affinity of the vector for the polymer, which depends upon the strength of molecular interactions. These interactions occur through nonspecific binding based on vector and polymer composition or through the incorporation of complementary binding sites (e.g., biotin-avidin). This review examines the delivery of nonviral and viral vectors from natural and synthetic polymers and presents opportunities for continuing developments to increase their applicability.

AB - Adapting controlled release technologies to the delivery of DNA has the potential to overcome extracellular barriers that limit gene therapy. Controlled release systems can enhance gene delivery and increase the extent and duration of transgene expression relative to more traditional delivery methods (e.g., injection). These systems typically deliver vectors locally, which can avoid distribution to distant tissues, decrease toxicity to nontarget cells, and reduce the immune response to the vector. Delivery vehicles for controlled release are fabricated from natural and synthetic polymers, which function either by releasing the vector into the local tissue environment or by maintaining the vector at the polymer surface. Vector release or binding is regulated by the effective affinity of the vector for the polymer, which depends upon the strength of molecular interactions. These interactions occur through nonspecific binding based on vector and polymer composition or through the incorporation of complementary binding sites (e.g., biotin-avidin). This review examines the delivery of nonviral and viral vectors from natural and synthetic polymers and presents opportunities for continuing developments to increase their applicability.

KW - Biomaterials

KW - Gene therapy

KW - Plasmid

KW - Substrate-mediated delivery

KW - Tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=3042814407&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3042814407&partnerID=8YFLogxK

U2 - 10.1016/j.ymthe.2004.03.020

DO - 10.1016/j.ymthe.2004.03.020

M3 - Review article

C2 - 15233938

AN - SCOPUS:3042814407

VL - 10

SP - 19

EP - 26

JO - Molecular Therapy

JF - Molecular Therapy

SN - 1525-0016

IS - 1

ER -