Controlled delivery of mesenchymal stem cells and growth factors using a nanofiber scaffold for tendon repair

C. N. Manning, A. G. Schwartz, W. Liu, J. Xie, N. Havlioglu, S. E. Sakiyama-Elbert, M. J. Silva, Y. Xia, R. H. Gelberman, S. Thomopoulos

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

Outcomes after tendon repair are often unsatisfactory, despite improvements in surgical techniques and rehabilitation methods. Recent studies aimed at enhancing repair have targeted the paucicellular nature of tendon for enhancing repair; however, most approaches for delivering growth factors and cells have not been designed for dense connective tissues such as tendon. Therefore, we developed a scaffold capable of delivering growth factors and cells in a surgically manageable form for tendon repair. Platelet-derived growth factor BB (PDGF-BB), along with adipose-derived mesenchymal stem cells (ASCs), were incorporated into a heparin/fibrin-based delivery system (HBDS). This hydrogel was then layered with an electrospun nanofiber poly(lactic-co-glycolic acid) (PLGA) backbone. The HBDS allowed for the concurrent delivery of PDGF-BB and ASCs in a controlled manner, while the PLGA backbone provided structural integrity for surgical handling and tendon implantation. In vitro studies verified that the cells remained viable, and that sustained growth factor release was achieved. In vivo studies in a large animal tendon model verified that the approach was clinically relevant, and that the cells remained viable in the tendon repair environment. Only a mild immunoresponse was seen at dissection, histologically, and at the mRNA level; fluorescently labeled ASCs and the scaffold were found at the repair site 9 days post-operatively; and increased total DNA was observed in ASC-treated tendons. The novel layered scaffold has the potential for improving tendon healing due to its ability to deliver both cells and growth factors simultaneously in a surgically convenient manner.

Original languageEnglish (US)
Pages (from-to)6905-6914
Number of pages10
JournalActa Biomaterialia
Volume9
Issue number6
DOIs
StatePublished - Jun 1 2013

Fingerprint

Nanofibers
Stem Cell Factor
Tendons
Stem cells
Mesenchymal Stromal Cells
Scaffolds
Intercellular Signaling Peptides and Proteins
Repair
Platelets
Fibrin
Heparin
Dissection
Acids
Hydrogel
Structural integrity
Hydrogels
Patient rehabilitation
Connective Tissue
Animals
DNA

Keywords

  • Electrospinning
  • Intrasynovial flexor tendon
  • Stem cells
  • Sustained delivery system
  • Tissue engineering

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering
  • Molecular Biology

Cite this

Manning, C. N., Schwartz, A. G., Liu, W., Xie, J., Havlioglu, N., Sakiyama-Elbert, S. E., ... Thomopoulos, S. (2013). Controlled delivery of mesenchymal stem cells and growth factors using a nanofiber scaffold for tendon repair. Acta Biomaterialia, 9(6), 6905-6914. https://doi.org/10.1016/j.actbio.2013.02.008

Controlled delivery of mesenchymal stem cells and growth factors using a nanofiber scaffold for tendon repair. / Manning, C. N.; Schwartz, A. G.; Liu, W.; Xie, J.; Havlioglu, N.; Sakiyama-Elbert, S. E.; Silva, M. J.; Xia, Y.; Gelberman, R. H.; Thomopoulos, S.

In: Acta Biomaterialia, Vol. 9, No. 6, 01.06.2013, p. 6905-6914.

Research output: Contribution to journalArticle

Manning, CN, Schwartz, AG, Liu, W, Xie, J, Havlioglu, N, Sakiyama-Elbert, SE, Silva, MJ, Xia, Y, Gelberman, RH & Thomopoulos, S 2013, 'Controlled delivery of mesenchymal stem cells and growth factors using a nanofiber scaffold for tendon repair', Acta Biomaterialia, vol. 9, no. 6, pp. 6905-6914. https://doi.org/10.1016/j.actbio.2013.02.008
Manning, C. N. ; Schwartz, A. G. ; Liu, W. ; Xie, J. ; Havlioglu, N. ; Sakiyama-Elbert, S. E. ; Silva, M. J. ; Xia, Y. ; Gelberman, R. H. ; Thomopoulos, S. / Controlled delivery of mesenchymal stem cells and growth factors using a nanofiber scaffold for tendon repair. In: Acta Biomaterialia. 2013 ; Vol. 9, No. 6. pp. 6905-6914.
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