Control of meristem development by CLAVATA1 receptor kinase and kinase-associated protein phosphatase interactions

Julie M. Stone, Amy E. Trotochaud, John C. Walker, Steven E. Clark

Research output: Contribution to journalArticle

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Abstract

The CLAVATA1 (CLV1) gene encodes a putative receptor kinase required for the proper balance between cell proliferation and differentiation in Arabidopsis shoot and flower meristems. Impaired CLV1 signaling results in masses of undifferentiated cells at the shoot and floral meristems. Although many putative receptor kinases have been identified in plants, the mechanism of signal transduction mediated by plant receptor-like kinases is largely unknown. One potential effector of receptor kinase signaling is kinase-associated protein phosphatase (KAPP), a protein that binds to multiple plant receptor-like kinases in a phosphorylation-dependent manner. To examine a possible role for KAPP in CLV1 -dependent plant development, the interaction of CLV1 and KAPP was investigated in vitro and in vivo. KAPP binds directly to autophosphorylated CLV1 in vitro and co-immunoprecipitates with CLV1 in plant extracts derived from meristematic tissue. Reduction of KAPP transcript accumulation in an intermediate clv1 mutant suppresses the mutant phenotype, and the degree of suppression is inversely correlated with KAPP mRNA levels. These data suggest that KAPP functions as a negative regulator of CLV1 signaling in plant development. This may represent a general model for the interaction of KAPP with receptor kinases.

Original languageEnglish (US)
Pages (from-to)1217-1225
Number of pages9
JournalPlant physiology
Volume117
Issue number4
DOIs
StatePublished - Jan 1 1998

Fingerprint

Meristem
Phosphoprotein Phosphatases
meristems
phosphotransferases (kinases)
Phosphotransferases
receptors
proteins
Plant Development
plant development
mutants
shoots
Plant Extracts
Arabidopsis
plant extracts
cell differentiation
signal transduction
Cell Differentiation
Signal Transduction
cell proliferation
phosphorylation

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Control of meristem development by CLAVATA1 receptor kinase and kinase-associated protein phosphatase interactions. / Stone, Julie M.; Trotochaud, Amy E.; Walker, John C.; Clark, Steven E.

In: Plant physiology, Vol. 117, No. 4, 01.01.1998, p. 1217-1225.

Research output: Contribution to journalArticle

Stone, Julie M. ; Trotochaud, Amy E. ; Walker, John C. ; Clark, Steven E. / Control of meristem development by CLAVATA1 receptor kinase and kinase-associated protein phosphatase interactions. In: Plant physiology. 1998 ; Vol. 117, No. 4. pp. 1217-1225.
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