Contribution of impaired myocardial insulin signaling to mitochondrial dysfunction and oxidative stress in the heart

Sihem Boudina, Heiko Bugger, Sandra Sena, Brian T. O'Neill, Vlad G. Zaha, Olesya Ilkun, Jordan J. Wright, Pradip K. Mazumder, Eric Palfreyman, Timothy J. Tidwell, Heather Theobald, Oleh Khalimonchuk, Benjamin Wayment, Xiaoming Sheng, Kenneth J. Rodnick, Ryan Centini, Dong Chen, Sheldon E. Litwin, Bart E. Weimer, E. Dale Abel

Research output: Contribution to journalArticle

183 Citations (Scopus)

Abstract

Background-Diabetes-associated cardiac dysfunction is associated with mitochondrial dysfunction and oxidative stress, which may contribute to left ventricular dysfunction. The contribution of altered myocardial insulin action, independent of associated changes in systemic metabolism, is incompletely understood. The present study tested the hypothesis that perinatal loss of insulin signaling in the heart impairs mitochondrial function. Methods and Results-In 8-week-old mice with cardiomyocyte deletion of insulin receptors (CIRKO), inotropic reserves were reduced, and mitochondria manifested respiratory defects for pyruvate that was associated with proportionate reductions in catalytic subunits of pyruvate dehydrogenase. Progressive age-dependent defects in oxygen consumption and ATP synthesis with the substrate glutamate and the fatty acid derivative palmitoyl-carnitine were observed. Mitochondria also were uncoupled when exposed to palmitoyl-carnitine, in part as a result of increased reactive oxygen species production and oxidative stress. Although proteomic and genomic approaches revealed a reduction in subsets of genes and proteins related to oxidative phosphorylation, no reductions in maximal activities of mitochondrial electron transport chain complexes were found. However, a disproportionate reduction in tricarboxylic acid cycle and fatty acid oxidation proteins in mitochondria suggests that defects in fatty acid and pyruvate metabolism and tricarboxylic acid flux may explain the mitochondrial dysfunction observed. Conclusions-Impaired myocardial insulin signaling promotes oxidative stress and mitochondrial uncoupling, which, together with reduced tricarboxylic acid and fatty acid oxidative capacity, impairs mitochondrial energetics. This study identifies specific contributions of impaired insulin action to mitochondrial dysfunction in the heart.

Original languageEnglish (US)
Pages (from-to)1272-1283
Number of pages12
JournalCirculation
Volume119
Issue number9
DOIs
StatePublished - Mar 10 2009

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Oxidative Stress
Fatty Acids
Tricarboxylic Acids
Pyruvic Acid
Insulin
Mitochondria
Carnitine
Citric Acid Cycle
Oxidative Phosphorylation
Insulin Receptor
Left Ventricular Dysfunction
Electron Transport
Cardiac Myocytes
Oxygen Consumption
Proteomics
Glutamic Acid
Reactive Oxygen Species
Catalytic Domain
Oxidoreductases
Proteins

Keywords

  • Insulin
  • Metabolism
  • Mitochondria
  • Oxidative stress

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Boudina, S., Bugger, H., Sena, S., O'Neill, B. T., Zaha, V. G., Ilkun, O., ... Abel, E. D. (2009). Contribution of impaired myocardial insulin signaling to mitochondrial dysfunction and oxidative stress in the heart. Circulation, 119(9), 1272-1283. https://doi.org/10.1161/CIRCULATIONAHA.108.792101

Contribution of impaired myocardial insulin signaling to mitochondrial dysfunction and oxidative stress in the heart. / Boudina, Sihem; Bugger, Heiko; Sena, Sandra; O'Neill, Brian T.; Zaha, Vlad G.; Ilkun, Olesya; Wright, Jordan J.; Mazumder, Pradip K.; Palfreyman, Eric; Tidwell, Timothy J.; Theobald, Heather; Khalimonchuk, Oleh; Wayment, Benjamin; Sheng, Xiaoming; Rodnick, Kenneth J.; Centini, Ryan; Chen, Dong; Litwin, Sheldon E.; Weimer, Bart E.; Abel, E. Dale.

In: Circulation, Vol. 119, No. 9, 10.03.2009, p. 1272-1283.

Research output: Contribution to journalArticle

Boudina, S, Bugger, H, Sena, S, O'Neill, BT, Zaha, VG, Ilkun, O, Wright, JJ, Mazumder, PK, Palfreyman, E, Tidwell, TJ, Theobald, H, Khalimonchuk, O, Wayment, B, Sheng, X, Rodnick, KJ, Centini, R, Chen, D, Litwin, SE, Weimer, BE & Abel, ED 2009, 'Contribution of impaired myocardial insulin signaling to mitochondrial dysfunction and oxidative stress in the heart', Circulation, vol. 119, no. 9, pp. 1272-1283. https://doi.org/10.1161/CIRCULATIONAHA.108.792101
Boudina, Sihem ; Bugger, Heiko ; Sena, Sandra ; O'Neill, Brian T. ; Zaha, Vlad G. ; Ilkun, Olesya ; Wright, Jordan J. ; Mazumder, Pradip K. ; Palfreyman, Eric ; Tidwell, Timothy J. ; Theobald, Heather ; Khalimonchuk, Oleh ; Wayment, Benjamin ; Sheng, Xiaoming ; Rodnick, Kenneth J. ; Centini, Ryan ; Chen, Dong ; Litwin, Sheldon E. ; Weimer, Bart E. ; Abel, E. Dale. / Contribution of impaired myocardial insulin signaling to mitochondrial dysfunction and oxidative stress in the heart. In: Circulation. 2009 ; Vol. 119, No. 9. pp. 1272-1283.
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AU - Boudina, Sihem

AU - Bugger, Heiko

AU - Sena, Sandra

AU - O'Neill, Brian T.

AU - Zaha, Vlad G.

AU - Ilkun, Olesya

AU - Wright, Jordan J.

AU - Mazumder, Pradip K.

AU - Palfreyman, Eric

AU - Tidwell, Timothy J.

AU - Theobald, Heather

AU - Khalimonchuk, Oleh

AU - Wayment, Benjamin

AU - Sheng, Xiaoming

AU - Rodnick, Kenneth J.

AU - Centini, Ryan

AU - Chen, Dong

AU - Litwin, Sheldon E.

AU - Weimer, Bart E.

AU - Abel, E. Dale

PY - 2009/3/10

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N2 - Background-Diabetes-associated cardiac dysfunction is associated with mitochondrial dysfunction and oxidative stress, which may contribute to left ventricular dysfunction. The contribution of altered myocardial insulin action, independent of associated changes in systemic metabolism, is incompletely understood. The present study tested the hypothesis that perinatal loss of insulin signaling in the heart impairs mitochondrial function. Methods and Results-In 8-week-old mice with cardiomyocyte deletion of insulin receptors (CIRKO), inotropic reserves were reduced, and mitochondria manifested respiratory defects for pyruvate that was associated with proportionate reductions in catalytic subunits of pyruvate dehydrogenase. Progressive age-dependent defects in oxygen consumption and ATP synthesis with the substrate glutamate and the fatty acid derivative palmitoyl-carnitine were observed. Mitochondria also were uncoupled when exposed to palmitoyl-carnitine, in part as a result of increased reactive oxygen species production and oxidative stress. Although proteomic and genomic approaches revealed a reduction in subsets of genes and proteins related to oxidative phosphorylation, no reductions in maximal activities of mitochondrial electron transport chain complexes were found. However, a disproportionate reduction in tricarboxylic acid cycle and fatty acid oxidation proteins in mitochondria suggests that defects in fatty acid and pyruvate metabolism and tricarboxylic acid flux may explain the mitochondrial dysfunction observed. Conclusions-Impaired myocardial insulin signaling promotes oxidative stress and mitochondrial uncoupling, which, together with reduced tricarboxylic acid and fatty acid oxidative capacity, impairs mitochondrial energetics. This study identifies specific contributions of impaired insulin action to mitochondrial dysfunction in the heart.

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KW - Insulin

KW - Metabolism

KW - Mitochondria

KW - Oxidative stress

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