Contrast expression patterns of JNK1 during sex reversal of the rice-field Eel

Ya Mei Xiao, Lili Chen, Jiao Liu, Wen Bin Liu, He Ge Chen, Li Jun Zou, Yun Liu, David Wan Cheng Li

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The c-Jun N-terminal kinases (JNKs) are members of the mitogen-activated protein kinase family. Their functions in regulating animal development have been well studied in both invertebrates and vertebrates. However, it remains to be determined whether they play a role in sex determination. Here we present first evidence to show that expression of JNK1 displays distinct patterns during sex reversal of rice-field eel. Molecular cloning reveals that JNK1 is well conserved among rice-field eel and other vertebrates. Both quantitative real-time polymerase chain reaction and Western blot analysis demonstrate that JNK1 is highly expressed in the ovary of the female individual and reduced to a substantial degree at the later stage of the intersex. However, when the intersex individual develops into the stage of male, expression of the JNK1 in the testis of the male individual is distinctly downregulated. Associated with the contrast JNK1 expression pattern in female and male gonads, several stem cell marker genes including Nanog, Oct-3/4, and Sox-2 were also differentially expressed in female and male germinal stem cells. Together, these results suggest it is possible that JNK1 plays an important role in sexual reversal of the rice-field eel.

Original languageEnglish (US)
Pages (from-to)242-256
Number of pages15
JournalJournal of Experimental Zoology Part B: Molecular and Developmental Evolution
Volume314 B
Issue number3
DOIs
StatePublished - May 15 2010

Fingerprint

Monopterus albus
sex reversal
Eels
eel
paddy field
intersex
mitogen-activated protein kinase
Vertebrates
stem cells
vertebrate
Stem Cells
vertebrates
animal development
sex determination
JNK Mitogen-Activated Protein Kinases
Gonads
Molecular Cloning
Invertebrates
Mitogen-Activated Protein Kinases
polymerase chain reaction

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Medicine
  • Animal Science and Zoology
  • Genetics
  • Developmental Biology

Cite this

Contrast expression patterns of JNK1 during sex reversal of the rice-field Eel. / Xiao, Ya Mei; Chen, Lili; Liu, Jiao; Liu, Wen Bin; Chen, He Ge; Zou, Li Jun; Liu, Yun; Li, David Wan Cheng.

In: Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, Vol. 314 B, No. 3, 15.05.2010, p. 242-256.

Research output: Contribution to journalArticle

Xiao, Ya Mei ; Chen, Lili ; Liu, Jiao ; Liu, Wen Bin ; Chen, He Ge ; Zou, Li Jun ; Liu, Yun ; Li, David Wan Cheng. / Contrast expression patterns of JNK1 during sex reversal of the rice-field Eel. In: Journal of Experimental Zoology Part B: Molecular and Developmental Evolution. 2010 ; Vol. 314 B, No. 3. pp. 242-256.
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