Continuous, real-time monitoring of cocaine in undiluted blood serum via a microfluidic, electrochemical aptamer-based sensor

James S. Swensen, Yi Xiao, Brian S. Ferguson, Arica A. Lubin, Rebecca Y. Lai, Alan J. Heeger, Kevin W. Plaxco, H. Tom Soh

Research output: Contribution to journalArticle

221 Citations (Scopus)

Abstract

The development of a biosensor system capable of continuous, real-time measurement of small-molecule analytes directly in complex, unprocessed aqueous samples has been a significant challenge, and successful implementation has been achieved for only a limited number of targets. Toward a general solution to this problem, we report here the Microfluidic Electrochemical Aptamer-based Sensor (MECAS) chip wherein we integrate target-specific DNA aptamers that fold, and thus generate an electrochemical signal, in response to the analyte with a microfluidic detection system. As a model, we demonstrate the continuous, real-time (∼1 min time resolution) detection of the small-molecule drug cocaine at near physiological, low micromolar concentrations directly in undiluted, otherwise unmodified blood serum. We believe our approach of integrating folding-based electrochemical sensors with miniaturized detection systems may lay the groundwork for the real-time, point-of-care detection of a wide variety of molecular targets.

Original languageEnglish (US)
Pages (from-to)4262-4266
Number of pages5
JournalJournal of the American Chemical Society
Volume131
Issue number12
DOIs
StatePublished - Apr 1 2009

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Microfluidics
Cocaine
Blood
Nucleotide Aptamers
Electrochemical sensors
Molecules
Monitoring
Sensors
Time measurement
Serum
Biosensors
DNA
Point-of-Care Systems
Biosensing Techniques
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Continuous, real-time monitoring of cocaine in undiluted blood serum via a microfluidic, electrochemical aptamer-based sensor. / Swensen, James S.; Xiao, Yi; Ferguson, Brian S.; Lubin, Arica A.; Lai, Rebecca Y.; Heeger, Alan J.; Plaxco, Kevin W.; Soh, H. Tom.

In: Journal of the American Chemical Society, Vol. 131, No. 12, 01.04.2009, p. 4262-4266.

Research output: Contribution to journalArticle

Swensen, James S. ; Xiao, Yi ; Ferguson, Brian S. ; Lubin, Arica A. ; Lai, Rebecca Y. ; Heeger, Alan J. ; Plaxco, Kevin W. ; Soh, H. Tom. / Continuous, real-time monitoring of cocaine in undiluted blood serum via a microfluidic, electrochemical aptamer-based sensor. In: Journal of the American Chemical Society. 2009 ; Vol. 131, No. 12. pp. 4262-4266.
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