Continuous chromogenic tissue factor assay: Comparison to clot-based assays and sensitivity established using pure tissue factor

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Abstract

The continuous chromogenic tissue factor assay has been compared to one-stage and two-stage coagulation assays, and its sensitivity has been established using purified human placental tissue factor. Loglog plots of the clot-times versus A405/min2 are linear over the mutually useful ranges of tissue factor concentration. Using human placental tissue factor reconstituted into phosphatidylserine-phosphatidylcholine (30:70) vesicles, the chromogenic assay quantitatively detected tissue factor in dilutions containing from 3 femtomoles to less than 10 attomoles of protein.

Original languageEnglish (US)
Pages (from-to)379-387
Number of pages9
JournalThrombosis Research
Volume47
Issue number4
DOIs
StatePublished - Aug 15 1987

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Thromboplastin
Phosphatidylserines
Phosphatidylcholines
Proteins

Keywords

  • chromogenic assay
  • coagulation assays
  • tissue factor

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Hematology

Cite this

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