Construction of an opal suppressor by oligonucleotide-directed mutagenesis of a Saccharomyces cerevisiae tRNATrp gene

A. L. Atkin, K. L. Roy, J. B. Bell

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Abstract

In vitro mutagenesis was used to create putative opal suppressor alleles of a tRNATrp gene of Saccharomyces cerevisiae. The construct with the requisite anticodon change did not result in an active suppressor, whereas when a second change was introduced into the portion of the gene encoding the intron, an active and specific opal suppressor was produced. We propose that the secondary structure of transcripts from the first mutant may prevent efficient pre-tRNA processing, whereas normal processing occurs with the double mutant.

Original languageEnglish (US)
Pages (from-to)4379-4383
Number of pages5
JournalMolecular and cellular biology
Volume10
Issue number8
DOIs
StatePublished - Jan 1 1990

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ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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