Construction of a Sequence-Defined Transposon Mutant Library in Staphylococcus aureus

Jennifer L. Endres, Vijaya Kumar Yajjala, Paul D. Fey, Kenneth W Bayles

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Transposon mutagenesis is one of the most widely used strategies to generate a large number of random mutations within a bacterial genome and then to precisely identify the mutated sites. The generation of sequence-defined transposon mutant libraries that are composed of a collection of different mutants, each containing a single transposon insertion mutation within nearly all of the nonessential genes within the genome, is a rapid and reliable way to enhance the study of gene function. In this chapter, we describe the process to generate a sequence-defined transposon mutant library in Staphylococcus aureus utilizing the mariner-based bursa aurealis transposon.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages29-37
Number of pages9
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume2016
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Fingerprint

Staphylococcus aureus
Bacterial Genomes
Insertional Mutagenesis
Mutagenesis
Genes
Genome
Mutation

Keywords

  • Random mutagenesis
  • Staphylococcus aureus
  • Transposon
  • bursa aurealis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Endres, J. L., Yajjala, V. K., Fey, P. D., & Bayles, K. W. (2019). Construction of a Sequence-Defined Transposon Mutant Library in Staphylococcus aureus. In Methods in Molecular Biology (pp. 29-37). (Methods in Molecular Biology; Vol. 2016). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9570-7_3

Construction of a Sequence-Defined Transposon Mutant Library in Staphylococcus aureus. / Endres, Jennifer L.; Yajjala, Vijaya Kumar; Fey, Paul D.; Bayles, Kenneth W.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 29-37 (Methods in Molecular Biology; Vol. 2016).

Research output: Chapter in Book/Report/Conference proceedingChapter

Endres, JL, Yajjala, VK, Fey, PD & Bayles, KW 2019, Construction of a Sequence-Defined Transposon Mutant Library in Staphylococcus aureus. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 2016, Humana Press Inc., pp. 29-37. https://doi.org/10.1007/978-1-4939-9570-7_3
Endres JL, Yajjala VK, Fey PD, Bayles KW. Construction of a Sequence-Defined Transposon Mutant Library in Staphylococcus aureus. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 29-37. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9570-7_3
Endres, Jennifer L. ; Yajjala, Vijaya Kumar ; Fey, Paul D. ; Bayles, Kenneth W. / Construction of a Sequence-Defined Transposon Mutant Library in Staphylococcus aureus. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 29-37 (Methods in Molecular Biology).
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