Construction and use of a template block for radial immunodiffusion

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Details for design and construction of template blocks for the increased sensitivity of radial immunodiffusion (RID) assays are described. The sensitivity of the template RID was 10-fold higher for quantifying albumin and 5-fold higher for IgG than the conventional RID with wells cut into the agarose. The system described in this communication resulted from the unit which provided the most consistent results from a variety of trials with different block thicknesses, chamber sizes and depths, and chamber arrangements.

Original languageEnglish (US)
Pages (from-to)165-168
Number of pages4
JournalAnalytical Biochemistry
Volume186
Issue number1
DOIs
StatePublished - Jan 1 1990

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Immunodiffusion
Sepharose
Albumins
Assays
Immunoglobulin G
Communication

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Construction and use of a template block for radial immunodiffusion. / McDonald, Thomas L; Weber, Annika; Thiele, Geoffrey Milton.

In: Analytical Biochemistry, Vol. 186, No. 1, 01.01.1990, p. 165-168.

Research output: Contribution to journalArticle

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