Construction and immunogenicity of recombinant Mycobacterium bovis BCG expressing GP5 and M protein of porcine reproductive respiratory syndrome virus

Reginaldo G. Bastos, Odir A. Dellagostin, Raul G Barletta, Alan R Doster, Eric Nelson, Fernando A. Osorio

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Mycobacterium bovis BCG was used to express a truncated form of GP5 (lacking the first 30 NH2-terminal residues) and M protein of porcine reproductive and respiratory syndrome virus (PRRSV). The PRRSV proteins were expressed in BCG under control of the mycobacterial hsp60 gene promoter either in the mycobacterial cytoplasm (BCGGP5cyt and BCGMcyt) or as MT19-fusion proteins on the mycobacterial surface (BCGGP5surf and BCGMsurf). Mice inoculated with BCGGP5surf and BCGMsurf developed antibodies against the viral proteins at 30 days post-inoculation (dpi) as detected by ELISA and Western blot. By 60 dpi, the animals developed titer of neutralizing antibodies of 8. A PRRSV-specific gamma interferon response was also detected in splenocytes of recombinant BCG-inoculated mice at 60 and 90 dpi. These results indicate that BCG was able to express antigens of PRRSV and elicit an immune response against the viral proteins in mice.

Original languageEnglish (US)
Pages (from-to)21-29
Number of pages9
JournalVaccine
Volume21
Issue number1-2
DOIs
StatePublished - Nov 22 2002

Fingerprint

Mycobacterium bovis BCG
Porcine reproductive and respiratory syndrome virus
Mycobacterium bovis
Porcine respiratory and reproductive syndrome virus
immune response
viruses
swine
viral proteins
Viral Proteins
mice
proteins
splenocytes
interferon-gamma
neutralizing antibodies
Neutralizing Antibodies
Western blotting
cytoplasm
promoter regions
Interferon-gamma
enzyme-linked immunosorbent assay

Keywords

  • GP5 and M PRRSV protein
  • PRRSV
  • Recombinant BCG

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Construction and immunogenicity of recombinant Mycobacterium bovis BCG expressing GP5 and M protein of porcine reproductive respiratory syndrome virus. / Bastos, Reginaldo G.; Dellagostin, Odir A.; Barletta, Raul G; Doster, Alan R; Nelson, Eric; Osorio, Fernando A.

In: Vaccine, Vol. 21, No. 1-2, 22.11.2002, p. 21-29.

Research output: Contribution to journalArticle

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