Construction and characterization of a chimeric virus (BIV/HIV-1) carrying the bovine immunodeficiency virus gag-pol gene

Goumin Chen, Shuhui Wang, Kun Xiong, Jinzhong Wang, Tao Ye, Wenping Dong, Qi Wang, Qimin Chen, Yunqi Geng, Charles Wood, Yi Zeng

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

HIV-1 HXB2 5'LTR region, most of BIV R29 gag-pol segment and HIV-1 HXB2 pol IN - 3'LTR region were respectively amplified. A chimeric clone, designated as pHBIV 3753 , was constructed by cloning three fragments sequentially into pUC18. MT4 cells were transfected with pHBIV 3753 . The replication and expressions of the chimeric virus (HBIV 3753 ) were monitored by RT activity and IFA. The results firstly demonstrated that it is possible to generate a new type of the BIV/HIV-1 chimeric virus containing BIV gag-pol gene.

Original languageEnglish (US)
Pages (from-to)123-125
Number of pages3
JournalAIDS
Volume16
Issue number1
DOIs
StatePublished - Jan 4 2002

Fingerprint

Bovine Immunodeficiency Virus
pol Genes
gag Genes
HIV-1
Viruses
Organism Cloning
Clone Cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

Cite this

Construction and characterization of a chimeric virus (BIV/HIV-1) carrying the bovine immunodeficiency virus gag-pol gene. / Chen, Goumin; Wang, Shuhui; Xiong, Kun; Wang, Jinzhong; Ye, Tao; Dong, Wenping; Wang, Qi; Chen, Qimin; Geng, Yunqi; Wood, Charles; Zeng, Yi.

In: AIDS, Vol. 16, No. 1, 04.01.2002, p. 123-125.

Research output: Contribution to journalArticle

Chen, G, Wang, S, Xiong, K, Wang, J, Ye, T, Dong, W, Wang, Q, Chen, Q, Geng, Y, Wood, C & Zeng, Y 2002, 'Construction and characterization of a chimeric virus (BIV/HIV-1) carrying the bovine immunodeficiency virus gag-pol gene', AIDS, vol. 16, no. 1, pp. 123-125. https://doi.org/10.1097/00002030-200201040-00016
Chen, Goumin ; Wang, Shuhui ; Xiong, Kun ; Wang, Jinzhong ; Ye, Tao ; Dong, Wenping ; Wang, Qi ; Chen, Qimin ; Geng, Yunqi ; Wood, Charles ; Zeng, Yi. / Construction and characterization of a chimeric virus (BIV/HIV-1) carrying the bovine immunodeficiency virus gag-pol gene. In: AIDS. 2002 ; Vol. 16, No. 1. pp. 123-125.
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