Constitutive activation of PI3K in oocyte induces ovarian granulosa cell tumors

So-Youn Kim, Katherine Ebbert, Marilia H. Cordeiro, Megan M. Romero, Kelly A. Whelan, Adrian A. Suarez, Teresa K. Woodruff, Takeshi Kurita

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Cell-cell interactions play crucial roles in the maintenance of tissue homeostasis, a loss of which often leads to varying diseases, including cancer. Here, we report that uncontrolled PI3K activity within oocytes irreversibly transforms granulosa cells (GC), causing GC tumors (GCT) through perturbed local cell communication. Previously, we reported reproductive phenotypes of transgenic mice, in which expression of constitutively active mutant PI3K was induced in primordial oocytes by Gdf9-iCre. The transgenic mice (Cre+) demonstrated severe ovarian phenotypes, including the overgrowth of excess ovarian follicles and anovulation. Surprisingly, the Cre+ mice became cachectic by postnatal day 80 due to bilateral GCT. Although GCT cells proliferated independently of oocytes, local interactions with mutant PI3Kpositive oocytes during early folliculogenesis were essential for the GC transformation. Growing GCT cells expressed high levels of inhibin βA and nuclear SMAD3, and the proliferation rate was positively correlated with a high activin A to inhibin A ratio. These results suggested that the tumor cells stimulated their growth through an activin A autocrine signaling pathway, a hypothesis confirmed by activin A secretion in cultured GCT cells, which proliferated in response. Although communication between the oocyte and surrounding somatic cells is critical for the normal development of ovarian follicles, perturbations in oocyte-GC communication during early folliculogenesis can induce GCT by activating an autocrine growth circuit program in GC.

Original languageEnglish (US)
Pages (from-to)3851-3861
Number of pages11
JournalCancer Research
Volume76
Issue number13
DOIs
StatePublished - Jul 1 2016
Externally publishedYes

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Granulosa Cell Tumor
Phosphatidylinositol 3-Kinases
Oocytes
Granulosa Cells
Cell Communication
Neoplasms
Ovarian Follicle
Transgenic Mice
Autocrine Communication
Cultured Tumor Cells
Anovulation
Phenotype
Growth
Homeostasis
Communication
Maintenance
activin A

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Kim, S-Y., Ebbert, K., Cordeiro, M. H., Romero, M. M., Whelan, K. A., Suarez, A. A., ... Kurita, T. (2016). Constitutive activation of PI3K in oocyte induces ovarian granulosa cell tumors. Cancer Research, 76(13), 3851-3861. https://doi.org/10.1158/0008-5472.CAN-15-3358

Constitutive activation of PI3K in oocyte induces ovarian granulosa cell tumors. / Kim, So-Youn; Ebbert, Katherine; Cordeiro, Marilia H.; Romero, Megan M.; Whelan, Kelly A.; Suarez, Adrian A.; Woodruff, Teresa K.; Kurita, Takeshi.

In: Cancer Research, Vol. 76, No. 13, 01.07.2016, p. 3851-3861.

Research output: Contribution to journalArticle

Kim, S-Y, Ebbert, K, Cordeiro, MH, Romero, MM, Whelan, KA, Suarez, AA, Woodruff, TK & Kurita, T 2016, 'Constitutive activation of PI3K in oocyte induces ovarian granulosa cell tumors', Cancer Research, vol. 76, no. 13, pp. 3851-3861. https://doi.org/10.1158/0008-5472.CAN-15-3358
Kim, So-Youn ; Ebbert, Katherine ; Cordeiro, Marilia H. ; Romero, Megan M. ; Whelan, Kelly A. ; Suarez, Adrian A. ; Woodruff, Teresa K. ; Kurita, Takeshi. / Constitutive activation of PI3K in oocyte induces ovarian granulosa cell tumors. In: Cancer Research. 2016 ; Vol. 76, No. 13. pp. 3851-3861.
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