Consequences of p53 Gene Expression by Adenovirus Vector on Cell Cycle Arrest and Apoptosis in Human Aortic Vascular Smooth Muscle Cells

Dai Katayose, Robert Wersto, Kenneth Cowan, Prem Seth

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

p53 shows its tumor suppresser activity by inducing cell cycle arrest and/or apoptosis of tumor cells and these activities are in part mediated by p21 cyclin-dependent kinase inhibitor (also called as WAF1, Cip1 and SDI1). Using human aortic vascular smooth muscle cells, here we demonstrate that adenovirus vector expressing p53-induced p21, cell cycle arrest at G1 and G2/M boundary, and accumulation of cells in G1 subgroup. However, adenovirus vector expressing p21 induced only G1 cell cycle arrest. The adenovirus vector expressing p53 was 200 times more cytotoxic to human aortic vascular smooth muscle cells than adenovirus vector expressing p21. These results suggest that adenovirus expressing p53 induces cytotoxicity in human vascular smooth muscle cells by apoptosis and this cytotoxicity can not be fully accounted by p21 induction.

Original languageEnglish (US)
Pages (from-to)446-451
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume215
Issue number2
DOIs
StatePublished - Jan 1 1995

Fingerprint

p53 Genes
Cell Cycle Checkpoints
Vascular Smooth Muscle
Adenoviridae
Gene expression
Smooth Muscle Myocytes
Muscle
Cells
Apoptosis
Gene Expression
G1 Phase Cell Cycle Checkpoints
G2 Phase Cell Cycle Checkpoints
Cyclin-Dependent Kinase Inhibitor p21
Cytotoxicity
Tumors
Neoplasms

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Consequences of p53 Gene Expression by Adenovirus Vector on Cell Cycle Arrest and Apoptosis in Human Aortic Vascular Smooth Muscle Cells. / Katayose, Dai; Wersto, Robert; Cowan, Kenneth; Seth, Prem.

In: Biochemical and Biophysical Research Communications, Vol. 215, No. 2, 01.01.1995, p. 446-451.

Research output: Contribution to journalArticle

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