Consecutive enzyme cascades: Complement activation at the cell surface triggers increased tissue factor activity

S. D. Carson, D. R. Johnson

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Complement activation at the cell surface initiates cell damage through a series of reactions occurring at the cell membrane and, after assembly of the terminal membrane attack complex, produces leakage of cytoplasmic contents from the cell. It has been documented that chemical or physical damage to cell membranes can cause a rapid increase in the expression of tissue factor procoagulant activity. In this study, antibody-mediated complement activation at the cell surface resulted in increased tissue factor activity, which correlated with cytolysis, as measured by 51-chromium release. Therefore, complement fixation on the cell surface can have a direct and immediate stimulatory effect on the coagulation cascade at the point of its initiation, with formation of a fibrin clot requiring only three consecutive proteolytic reactions after immunologically mediated cell damage.

Original languageEnglish (US)
Pages (from-to)361-367
Number of pages7
JournalBlood
Volume76
Issue number2
StatePublished - Jan 1 1990

Fingerprint

Complement Activation
Thromboplastin
Chemical activation
Cell membranes
Enzymes
Cells
Complement Membrane Attack Complex
Chromium
Coagulation
Fibrin
Cell Membrane
Antibodies

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Consecutive enzyme cascades : Complement activation at the cell surface triggers increased tissue factor activity. / Carson, S. D.; Johnson, D. R.

In: Blood, Vol. 76, No. 2, 01.01.1990, p. 361-367.

Research output: Contribution to journalArticle

@article{27ff6eb80e6d42f4b15fa40a2914454a,
title = "Consecutive enzyme cascades: Complement activation at the cell surface triggers increased tissue factor activity",
abstract = "Complement activation at the cell surface initiates cell damage through a series of reactions occurring at the cell membrane and, after assembly of the terminal membrane attack complex, produces leakage of cytoplasmic contents from the cell. It has been documented that chemical or physical damage to cell membranes can cause a rapid increase in the expression of tissue factor procoagulant activity. In this study, antibody-mediated complement activation at the cell surface resulted in increased tissue factor activity, which correlated with cytolysis, as measured by 51-chromium release. Therefore, complement fixation on the cell surface can have a direct and immediate stimulatory effect on the coagulation cascade at the point of its initiation, with formation of a fibrin clot requiring only three consecutive proteolytic reactions after immunologically mediated cell damage.",
author = "Carson, {S. D.} and Johnson, {D. R.}",
year = "1990",
month = "1",
day = "1",
language = "English (US)",
volume = "76",
pages = "361--367",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "2",

}

TY - JOUR

T1 - Consecutive enzyme cascades

T2 - Complement activation at the cell surface triggers increased tissue factor activity

AU - Carson, S. D.

AU - Johnson, D. R.

PY - 1990/1/1

Y1 - 1990/1/1

N2 - Complement activation at the cell surface initiates cell damage through a series of reactions occurring at the cell membrane and, after assembly of the terminal membrane attack complex, produces leakage of cytoplasmic contents from the cell. It has been documented that chemical or physical damage to cell membranes can cause a rapid increase in the expression of tissue factor procoagulant activity. In this study, antibody-mediated complement activation at the cell surface resulted in increased tissue factor activity, which correlated with cytolysis, as measured by 51-chromium release. Therefore, complement fixation on the cell surface can have a direct and immediate stimulatory effect on the coagulation cascade at the point of its initiation, with formation of a fibrin clot requiring only three consecutive proteolytic reactions after immunologically mediated cell damage.

AB - Complement activation at the cell surface initiates cell damage through a series of reactions occurring at the cell membrane and, after assembly of the terminal membrane attack complex, produces leakage of cytoplasmic contents from the cell. It has been documented that chemical or physical damage to cell membranes can cause a rapid increase in the expression of tissue factor procoagulant activity. In this study, antibody-mediated complement activation at the cell surface resulted in increased tissue factor activity, which correlated with cytolysis, as measured by 51-chromium release. Therefore, complement fixation on the cell surface can have a direct and immediate stimulatory effect on the coagulation cascade at the point of its initiation, with formation of a fibrin clot requiring only three consecutive proteolytic reactions after immunologically mediated cell damage.

UR - http://www.scopus.com/inward/record.url?scp=0025330312&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025330312&partnerID=8YFLogxK

M3 - Article

C2 - 2369639

AN - SCOPUS:0025330312

VL - 76

SP - 361

EP - 367

JO - Blood

JF - Blood

SN - 0006-4971

IS - 2

ER -