Conjunctivitis caused by a swine Chlamydia trachomatis-like organism in gnotobiotic pigs

Douglas G. Rogers, Arthur A. Andersen

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The objective of this study was to determine whether a chlamydial strain recovered from growing and finishing swine with conjunctivitis or keratoconjunctivitis could cause the same infections in gnotobiotic pigs. The strain shares biological characteristics with Chlamydia trachomatis. After propagation in Vero cells and preparation of the inoculum (107 inclusion-forming units/ml), chlamydial strain H7 was instilled into the ventral conjunctival sac (0.15 ml/sac) of 12 anesthetized 3-day-old gnotobiotic piglets. Four age-matched gnotobiotic piglets were anesthetized and sham infected with uninfected cell culture lysates. None of the principal piglets developed clinical symptoms of conjunctivitis or keratoconjunctivitis. Principal piglets necropsied 7 days postinfection (DPI) had histologic lesions of mild or moderate conjunctivitis; immunohistochemical evaluation revealed chlamydial antigen in conjunctival epithelium. A majority of principal piglets necropsied at 14-28 DPI had histologic lesions of mild conjunctivitis, but chlamydial antigen was not detected by immunohistochemistry. The results indicated that chlamydial strain H7 can cause mild or occasionally moderate conjunctivitis in gnotobiotic pigs, but the conjunctival infection is asymptomatic.

Original languageEnglish (US)
Pages (from-to)341-344
Number of pages4
JournalJournal of Veterinary Diagnostic Investigation
Volume11
Issue number4
DOIs
StatePublished - Jul 1999

Fingerprint

Chlamydia trachomatis
Germ-Free Life
conjunctivitis
Conjunctivitis
piglets
Swine
swine
organisms
Keratoconjunctivitis
keratoconjunctivitis
lesions (animal)
lacrimal apparatus
antigens
Antigens
Lacrimal Apparatus
Asymptomatic Infections
Vero Cells
infection
signs and symptoms (animals and humans)
immunohistochemistry

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Conjunctivitis caused by a swine Chlamydia trachomatis-like organism in gnotobiotic pigs. / Rogers, Douglas G.; Andersen, Arthur A.

In: Journal of Veterinary Diagnostic Investigation, Vol. 11, No. 4, 07.1999, p. 341-344.

Research output: Contribution to journalArticle

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