Confocal fluorescence microscopy: An ultra-sensitive tool used to evaluate intracellular antiretroviral nano-drug delivery in HeLa cells

Subhra Mandal, You Zhou, Annemarie Shibata, Christopher J. Destache

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In the last decade, confocal fluorescence microscopy has emerged as an ultra-sensitive tool for real-time study of nanoparticles (NPs) fate at the cellular-level. According to WHO 2007 report, Human Immunodeficiency Virus/Acquired Immunodeficiency Syndrome (HIV/AIDS) is still one of the world's major health threats by claiming approximately 7,000 new infections daily worldwide. Although combination antiretroviral drugs (cARV) therapy has improved the life-expectancy of HIV-infected patients, routine use of high doses of cARV has serious health consequences and requires complete adherence to the regimen for success. Thus, our research goal is to fabricate long-acting novel cARV loaded poly(lactide-co-glycolic acid) (PLGA) nanoparticles (cARV-NPs) as drug delivery system. However, important aspects of cARV-NPs that require special emphasis are their cellular-uptake, potency, and sustained drug release efficiency over-time. In this article, ultra-sensitive confocal microscopy is been used to evaluate the uptake and sustained drug release kinetics of cARV-NPs in HeLa cells. To evaluate with the above goal, instead of cARV-drug, Rhodamine6G dye (fluorescent dye) loaded NPs (Rho6G NPs) have been formulated. To correlate the Rhodamin6G release kinetics with the ARV release from NPs, a parallel HPLC study was also performed. The results obtained indicate that Rho6G NPs were efficiently taken up at low concentration (<500 ng/ml) and that release was sustained for a minimum of 4 days of treatment. Therefore, high drug assimilation and sustained release properties of PLGA-NPs make them an attractive vehicle for cARV nano-drug delivery with the potential to reduce drug dosage as well as the number of drug administrations per month.

Original languageEnglish (US)
Article number084803
JournalAIP Advances
Volume5
Issue number8
DOIs
StatePublished - Aug 1 2015

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delivery
drugs
microscopy
fluorescence
cells
nanoparticles
human immunodeficiency virus
health
dyes
acquired immunodeficiency syndrome
dosage
acids
kinetics
assimilation
infectious diseases
chemotherapy
low concentrations
vehicles

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Confocal fluorescence microscopy : An ultra-sensitive tool used to evaluate intracellular antiretroviral nano-drug delivery in HeLa cells. / Mandal, Subhra; Zhou, You; Shibata, Annemarie; Destache, Christopher J.

In: AIP Advances, Vol. 5, No. 8, 084803, 01.08.2015.

Research output: Contribution to journalArticle

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