Computational modeling and functional characterization of a GgChi: A class III chitinase from corms of Gladiolus grandiflorus

Maria Rafiq, Ashiq Hussain, Kausar Hussain Shah, Qamar Saeed, Muhammad Umair Sial, Zahid Ali, Friedrich Buck, Richard E. Goodman, Binish Khaliq, Uzma Ishaq, Mirza Ahsen Baig, Aisha Munawar, Seema Mahmood, Ahmed Akrem

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The present study describes the predicted model and functional characterization of an endochitinase (30 kDa) from corms of Gladiolus grandiflorus. ESI-QTOF-MS generated peptide showed 96% sequence homology with family 18, Class III acidic endochitinase of Gladiolus gandavensis. Purified G. grandiflorus chitinase (GgChi) hydrolyzed 4-methylumbelliferyl β-D-N,N′,N′′-triacetylchitotriose substrate showing specific endochitinase activity. Since no structural details of GgChi were available in the Protein Data Bank (PDB), a homology model was predicted using the coordinate information of Crocus vernus chitinase (PDB ID: 3SIM). Ramachandran plot indicated 84.5% in most favored region, 14.8% in additional and 0.6% in generously allowed region while no residue in disallowed region. The predicted structure indicated a highly conserved (β/α) 8 (TIM barrel) structure similar to the family 18, class III chitinases. The GgChi also showed sequence and structural homologies with other active chitinases. The GgChi (50 μg/disc) showed no antibacterial activity, but did provide mild growth inhibition of phytopathogenic fungus Fusarium oxysporum at a concentration of 500 μg/well Similarly, insect toxicity bioassays of GgChi (50 μg) against nymphs of Bemisia tabaci showed 14% reduction in adult emergence and 14% increase in mortality rate in comparison to control values. The GgChi (1.5 mg) protein showed significant reduction in a population of flour beetle (Tribolium castaneum) after 35 days, but lower reactivity against rice weevil (Sitophilus oryzae). The results of this study provide detai.led insight on functional characterization of a family 18 class III acidic plant endochitinase.

Original languageEnglish (US)
Pages (from-to)673-683
Number of pages11
JournalKaohsiung Journal of Medical Sciences
Volume34
Issue number12
DOIs
StatePublished - Dec 2018

Fingerprint

Chitinases
Sequence Homology
class III chitinase
Databases
Tribolium
Weevils
Nymph
Proteins
Beetles
Fusarium
Flour
Biological Assay
Insects
Fungi

Keywords

  • Antibacterial
  • Antifungal
  • Endochitinase
  • Gladiolus grandiflorus
  • TIM barrel

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Computational modeling and functional characterization of a GgChi : A class III chitinase from corms of Gladiolus grandiflorus. / Rafiq, Maria; Hussain, Ashiq; Shah, Kausar Hussain; Saeed, Qamar; Sial, Muhammad Umair; Ali, Zahid; Buck, Friedrich; Goodman, Richard E.; Khaliq, Binish; Ishaq, Uzma; Baig, Mirza Ahsen; Munawar, Aisha; Mahmood, Seema; Akrem, Ahmed.

In: Kaohsiung Journal of Medical Sciences, Vol. 34, No. 12, 12.2018, p. 673-683.

Research output: Contribution to journalArticle

Rafiq, M, Hussain, A, Shah, KH, Saeed, Q, Sial, MU, Ali, Z, Buck, F, Goodman, RE, Khaliq, B, Ishaq, U, Baig, MA, Munawar, A, Mahmood, S & Akrem, A 2018, 'Computational modeling and functional characterization of a GgChi: A class III chitinase from corms of Gladiolus grandiflorus', Kaohsiung Journal of Medical Sciences, vol. 34, no. 12, pp. 673-683. https://doi.org/10.1016/j.kjms.2018.08.003
Rafiq, Maria ; Hussain, Ashiq ; Shah, Kausar Hussain ; Saeed, Qamar ; Sial, Muhammad Umair ; Ali, Zahid ; Buck, Friedrich ; Goodman, Richard E. ; Khaliq, Binish ; Ishaq, Uzma ; Baig, Mirza Ahsen ; Munawar, Aisha ; Mahmood, Seema ; Akrem, Ahmed. / Computational modeling and functional characterization of a GgChi : A class III chitinase from corms of Gladiolus grandiflorus. In: Kaohsiung Journal of Medical Sciences. 2018 ; Vol. 34, No. 12. pp. 673-683.
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AU - Goodman, Richard E.

AU - Khaliq, Binish

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