Comprehensive analysis of the Brassica juncea root proteome in response to cadmium exposure by complementary proteomic approaches

Sophie Alvarez, Bertram M. Berla, Jeanne Sheffield, Rebecca E. Cahoon, Joseph M. Jez, Leslie M. Hicks

Research output: Contribution to journalArticle

Abstract

Indian mustard (Brassicajuncea L.) is known to both accumulate and tolerate high levels of heavy metals from polluted soils. To gain a comprehensive understanding of the effect of cadmium (Cd) treatment on B. juncea roots, two quantitative proteomics approaches - fluorescence two- dimensional difference gel electrophoresis (2-D DIGE) and multiplexed isobaric tagging technology (iTRAQ) - were implemented. Several proteins involved in sulfur assimilation, redox homeostasis, and xenobiotic detoxification were found to be up-regulated. Multiple proteins involved in protein synthesis and processing were down-regulated. While the two proteomics approaches identified different sets of proteins, the proteins identified in both datasets are involved in similar biological processes. We show that 2-D DIGE and iTRAQ results are complementary, that the data obtained independently using the two techniques validate one another, and that the quality of iTRAQ results depends on both the number of biological replicates and the number of sample injections. This study determined the involvement of enzymes such as peptide methionine sulfoxide reductase and 2-nitropropane dioxygenase in alternatives redox- regulation mechanisms, as well as O-acetylserine sulfhydrylase, glutathione-S-transferase and glutathione-conjugate membrane transporter, as essential players in the Cd hyperaccumation and tolerance ofB. juncea.

Original languageEnglish (US)
Pages (from-to)2419-2431
Number of pages13
JournalProteomics
Volume9
Issue number9
DOIs
StatePublished - May 1 2009

Fingerprint

Mustard Plant
Proteome
Cadmium
Proteomics
Proteins
Oxidation-Reduction
Cysteine Synthase
Two-Dimensional Difference Gel Electrophoresis
Biological Phenomena
Detoxification
Membrane Transport Proteins
Xenobiotics
Heavy Metals
Glutathione Transferase
Electrophoresis
Sulfur
Glutathione
Homeostasis
Soil
Fluorescence

Keywords

  • Brassica juncea
  • Cadmium
  • DIGE
  • Root proteome
  • iTRAQ

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Comprehensive analysis of the Brassica juncea root proteome in response to cadmium exposure by complementary proteomic approaches. / Alvarez, Sophie; Berla, Bertram M.; Sheffield, Jeanne; Cahoon, Rebecca E.; Jez, Joseph M.; Hicks, Leslie M.

In: Proteomics, Vol. 9, No. 9, 01.05.2009, p. 2419-2431.

Research output: Contribution to journalArticle

Alvarez, Sophie ; Berla, Bertram M. ; Sheffield, Jeanne ; Cahoon, Rebecca E. ; Jez, Joseph M. ; Hicks, Leslie M. / Comprehensive analysis of the Brassica juncea root proteome in response to cadmium exposure by complementary proteomic approaches. In: Proteomics. 2009 ; Vol. 9, No. 9. pp. 2419-2431.
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