Complicated urinary tract infection is associated with uroepithelial expression of proinflammatory protein S100A8

Leticia Reyes, Sophie Alvarez, Ayman Allam, Mary Reinhard, Mary B. Brown

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

F344 rats chronically infected with Ureaplasma parvum develop two distinct profiles: asymptomatic urinary tract infection (UTI) and UTI complicated by struvite urolithiasis. To identify factors that affect disease outcome, we characterized the temporal host immune response during infection by histopathologic analysis and in situ localization of U. parvum. We also used differential quantitative proteomics to identify distinguishing host cellular responses associated with complicated UTI. In animals in which microbial colonization was limited to the mucosal surface, inflammation was indistinguishable from that which occurred in sham-inoculated controls, and the inflammation resolved by 72 h postinoculation (p.i.) in both groups. However, inflammation persisted in animals with microbial colonization that extended into the deeper layers of the submucosa. Proteome profiling showed that bladder tissues from animals with complicated UTIs had significant increases (P < 0.01) in proteins involved in apoptosis, oxidative stress, and inflammation. Animals with complicated UTIs (2 weeks p.i.) had the highest concentrations of the proinflammatory protein S100A8 (P ≤ 0.005) in bladder tissues, and the levels of S100A8 positively correlated with those of proinflammatory cytokines GRO/KC (P ≤ 0.003) and interleukin-1α (P ≤ 0.03) in urine. The bladder uroepithelium was a prominent cell source of S100A8-S100A9 in animals with complicated UTIs (2 weeks p.i.), which was not detected in animals with asymptomatic UTIs (2 weeks p.i.) or in any bladder tissues harvested at earlier p.i. time points. Based on these results, we surmise that invasive colonization of the bladder triggers chronic inflammation and immune dysregulation, which may be critical to struvite formation.

Original languageEnglish (US)
Pages (from-to)4265-4274
Number of pages10
JournalInfection and immunity
Volume77
Issue number10
DOIs
StatePublished - Oct 1 2009

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Calgranulin A
Urinary Tract Infections
Urinary Bladder
Inflammation
Ureaplasma
Urolithiasis
Inbred F344 Rats
Proteome
Interleukin-1
Proteomics
Oxidative Stress
Urine
Apoptosis
Cytokines
Infection

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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Complicated urinary tract infection is associated with uroepithelial expression of proinflammatory protein S100A8. / Reyes, Leticia; Alvarez, Sophie; Allam, Ayman; Reinhard, Mary; Brown, Mary B.

In: Infection and immunity, Vol. 77, No. 10, 01.10.2009, p. 4265-4274.

Research output: Contribution to journalArticle

Reyes, Leticia ; Alvarez, Sophie ; Allam, Ayman ; Reinhard, Mary ; Brown, Mary B. / Complicated urinary tract infection is associated with uroepithelial expression of proinflammatory protein S100A8. In: Infection and immunity. 2009 ; Vol. 77, No. 10. pp. 4265-4274.
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