Complement levels in acute inflammatory lung injury

E. Schmid, Larry D Crouch, H. P. Friedl, N. M. Bless, G. O. Till, P. A. Ward

Research output: Contribution to journalArticle

Abstract

After systemic complement activation by cobra venom factor (CVF), which is known to produce a neutrophil dependent lung injury, the time course in the appearance of C5a in the serum of male Long Evans rats was measured over 30 minutes by ELISA. The effect of murine C5a on neutrophil chemotaxis and its inhibiton by a polyclonal rabbit anti rat antibody to C5a were also studied. High levels of C5a were reached as early as 1 minute after injection of CVF and remained elevated up to 30 minutes. The levels of C5a showed a dose response related to the dose of CVF injected. The chemotactic activity of C5a was inhibited by anti C5a whereas serum hemolytic complement activity (CH50 value) was not diminished by the antibody, suggesting that anti-C5a is not reactive with intact C5. The early appearance of C5a in the inflammatory process, its high chemotactic activity and its inhibition by anti C5a suggests that C5a plays a key role in acute inflammation that occurs as a result of complement activation.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number6
StatePublished - Dec 1 1996

Fingerprint

Elapidae
Acute Lung Injury
venoms
complement
Complement Activation
lungs
neutrophils
Rats
Anti-Idiotypic Antibodies
Neutrophils
Chemical activation
Long Evans Rats
antibodies
chemotaxis
rats
Lung Injury
Chemotaxis
Serum
dose response
Complement System Proteins

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Schmid, E., Crouch, L. D., Friedl, H. P., Bless, N. M., Till, G. O., & Ward, P. A. (1996). Complement levels in acute inflammatory lung injury. FASEB Journal, 10(6).

Complement levels in acute inflammatory lung injury. / Schmid, E.; Crouch, Larry D; Friedl, H. P.; Bless, N. M.; Till, G. O.; Ward, P. A.

In: FASEB Journal, Vol. 10, No. 6, 01.12.1996.

Research output: Contribution to journalArticle

Schmid, E, Crouch, LD, Friedl, HP, Bless, NM, Till, GO & Ward, PA 1996, 'Complement levels in acute inflammatory lung injury', FASEB Journal, vol. 10, no. 6.
Schmid E, Crouch LD, Friedl HP, Bless NM, Till GO, Ward PA. Complement levels in acute inflammatory lung injury. FASEB Journal. 1996 Dec 1;10(6).
Schmid, E. ; Crouch, Larry D ; Friedl, H. P. ; Bless, N. M. ; Till, G. O. ; Ward, P. A. / Complement levels in acute inflammatory lung injury. In: FASEB Journal. 1996 ; Vol. 10, No. 6.
@article{ade6ccf67eb24323bada0052b6407593,
title = "Complement levels in acute inflammatory lung injury",
abstract = "After systemic complement activation by cobra venom factor (CVF), which is known to produce a neutrophil dependent lung injury, the time course in the appearance of C5a in the serum of male Long Evans rats was measured over 30 minutes by ELISA. The effect of murine C5a on neutrophil chemotaxis and its inhibiton by a polyclonal rabbit anti rat antibody to C5a were also studied. High levels of C5a were reached as early as 1 minute after injection of CVF and remained elevated up to 30 minutes. The levels of C5a showed a dose response related to the dose of CVF injected. The chemotactic activity of C5a was inhibited by anti C5a whereas serum hemolytic complement activity (CH50 value) was not diminished by the antibody, suggesting that anti-C5a is not reactive with intact C5. The early appearance of C5a in the inflammatory process, its high chemotactic activity and its inhibition by anti C5a suggests that C5a plays a key role in acute inflammation that occurs as a result of complement activation.",
author = "E. Schmid and Crouch, {Larry D} and Friedl, {H. P.} and Bless, {N. M.} and Till, {G. O.} and Ward, {P. A.}",
year = "1996",
month = "12",
day = "1",
language = "English (US)",
volume = "10",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "6",

}

TY - JOUR

T1 - Complement levels in acute inflammatory lung injury

AU - Schmid, E.

AU - Crouch, Larry D

AU - Friedl, H. P.

AU - Bless, N. M.

AU - Till, G. O.

AU - Ward, P. A.

PY - 1996/12/1

Y1 - 1996/12/1

N2 - After systemic complement activation by cobra venom factor (CVF), which is known to produce a neutrophil dependent lung injury, the time course in the appearance of C5a in the serum of male Long Evans rats was measured over 30 minutes by ELISA. The effect of murine C5a on neutrophil chemotaxis and its inhibiton by a polyclonal rabbit anti rat antibody to C5a were also studied. High levels of C5a were reached as early as 1 minute after injection of CVF and remained elevated up to 30 minutes. The levels of C5a showed a dose response related to the dose of CVF injected. The chemotactic activity of C5a was inhibited by anti C5a whereas serum hemolytic complement activity (CH50 value) was not diminished by the antibody, suggesting that anti-C5a is not reactive with intact C5. The early appearance of C5a in the inflammatory process, its high chemotactic activity and its inhibition by anti C5a suggests that C5a plays a key role in acute inflammation that occurs as a result of complement activation.

AB - After systemic complement activation by cobra venom factor (CVF), which is known to produce a neutrophil dependent lung injury, the time course in the appearance of C5a in the serum of male Long Evans rats was measured over 30 minutes by ELISA. The effect of murine C5a on neutrophil chemotaxis and its inhibiton by a polyclonal rabbit anti rat antibody to C5a were also studied. High levels of C5a were reached as early as 1 minute after injection of CVF and remained elevated up to 30 minutes. The levels of C5a showed a dose response related to the dose of CVF injected. The chemotactic activity of C5a was inhibited by anti C5a whereas serum hemolytic complement activity (CH50 value) was not diminished by the antibody, suggesting that anti-C5a is not reactive with intact C5. The early appearance of C5a in the inflammatory process, its high chemotactic activity and its inhibition by anti C5a suggests that C5a plays a key role in acute inflammation that occurs as a result of complement activation.

UR - http://www.scopus.com/inward/record.url?scp=33748898309&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33748898309&partnerID=8YFLogxK

M3 - Article

VL - 10

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 6

ER -