Complement component 3 interactions with Coxsackievirus B3 capsid proteins

Innate immunity and the rapid formation of splenic antiviral germinal centers

Daniel R Anderson, C. M. Carthy, J. E. Wilson, D. Yang, D. V. Devine, B. M. McManus

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Innate immunity is central to the clearance of pathogens from hosts as well as to the definition of acquired immune responses (D. T. Fearon, and R. M. Locksley, Science 272:50-53, 1996). Coxsackievirus B3 (CVB3), a human cardiopathic virus, was evaluated for the ability to activate the alternative and classical pathway of complement. CVB3 proteins interact with complement component 3 (C3, a soluble protein effector of innate immunity) after either in vitro exposure to mouse serum or in vivo murine infection and activate the alternative pathway of complement. In addition, we demonstrate that vital antigen retention and localization in germinal centers is dependent on C3, while virus antigen retention in extrafollicular regions in the spleen is not. In vivo depletion of native C3 abolished the rapid formation of virus- specific germinal centers (by day 3 post-CVB3 infection) in the absence of serum anti-CVB3 antibodies. These studies demonstrate that innate immune mechanisms, such as C3 interaction with CVB3, are essential for splenic antiviral germinal center formation in naive (antigen nonsensitized) mice resistant (C57BL/6J strain) and susceptible (A/J strain) to CVB3-induced myocarditis.

Original languageEnglish (US)
Pages (from-to)8841-8845
Number of pages5
JournalJournal of virology
Volume71
Issue number11
StatePublished - Oct 28 1997

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Enterovirus
Complement C3
Germinal Center
Capsid Proteins
coat proteins
Innate Immunity
Antiviral Agents
complement
Alternative Complement Pathway
Viruses
Antigens
antigens
Coxsackievirus Infections
mice
Classical Complement Pathway
Myocarditis
vertebrate viruses
viruses
myocarditis
Serum

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Complement component 3 interactions with Coxsackievirus B3 capsid proteins : Innate immunity and the rapid formation of splenic antiviral germinal centers. / Anderson, Daniel R; Carthy, C. M.; Wilson, J. E.; Yang, D.; Devine, D. V.; McManus, B. M.

In: Journal of virology, Vol. 71, No. 11, 28.10.1997, p. 8841-8845.

Research output: Contribution to journalArticle

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