Competitive binding analysis of monoclonal antibodies reactive with porcine alveolar macrophages using anti-CD14 and anti-CD18

Tammy L Kielian, D. S. McVey, W. C. Davis, Y. B. Kim, F. Blecha

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Four monoclonal antibodies (mAb) from the myeloid subset panel of the First International Swine CD Workshop (74-22-15, DH59B, PM16-6, and MUC21A) were analyzed using competitive inhibition studies with anti-human CD14 (My4) and anti-human/anti-porcine CD18 (MHM23) on porcine alveolar macrophages. Results suggested that none of the mAb tested recognized CD14 or CD18 on porcine alveolar macrophages. Additionally, the cross-reactivity of My4 with porcine alveolar macrophages was established.

Original languageEnglish (US)
Pages (from-to)273-278
Number of pages6
JournalVeterinary Immunology and Immunopathology
Volume43
Issue number1-3
DOIs
StatePublished - Jan 1 1994

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Competitive Binding
Alveolar Macrophages
monoclonal antibodies
macrophages
Swine
Monoclonal Antibodies
swine
cross reaction
Education

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Immunology
  • veterinary(all)

Cite this

Competitive binding analysis of monoclonal antibodies reactive with porcine alveolar macrophages using anti-CD14 and anti-CD18. / Kielian, Tammy L; McVey, D. S.; Davis, W. C.; Kim, Y. B.; Blecha, F.

In: Veterinary Immunology and Immunopathology, Vol. 43, No. 1-3, 01.01.1994, p. 273-278.

Research output: Contribution to journalArticle

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