Comparison of Uroplakin Expression During Urothelial Carcinogenesis Induced by N-Butyl-N-(4-Hydroxybutyl)Nitrosamine in Rats and Mice

Kumiko Ogawa, Margaret St. John, Maria Luiza De Oliveira, Lora L Arnold, Tomoyuki Shirai, Tung Tien Sun, Samuel Monroe Cohen

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The expression of uroplakins, the tissue-specific and differentiation-dependent membrane proteins of the urothelium, was analyzed immunohistochemically in N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN)-treated rats and mice during bladder carcinogenesis. Male Fischer 344 rats were treated with 0.05% BBN in the drinking water for 10 wk and were euthanatized at week 20 of the experiment. BBN was administered to male B6D2F1 mice; it was either provided at a rate of 0.05% in the drinking water (for 26 wk) or 5 mg BBN was administered by intragastric gavage twice weekly for 10 wk, followed by 20 wk without treatment. In rats, BBN-induced, noninvasive, low-grade, papillary, transitional cell carcinoma (TCC) showed decreased uroplakin-staining of cells lining the lumen but showed increased expression in some nonluminal cells. In mice, nonpapillary, high-grade dysplasia, carcinoma in situ, and invasive carcinoma were induced. There was a marked decrease in the number of uroplakin-positive cells lining the lumen and in nonluminal cells. This occurred in normal-appearing urothelium in BBN-treated mice and in dysplasic urothelium, in carcinoma in situ, and in invasive TCC. The percentage of uroplakin-positive nonluminal cells was higher in control mice than in rats, but it was lower in the mouse than in the rat after BBN treatment. Uroplakin expression was disorderly and focal in BBN-treated urothelium in both species. These results indicate that BBN treatment changed the expression of uroplakins during bladder carcinogenesis, with differences in rats and mice being related to degree of tumor differentiation.

Original languageEnglish (US)
Pages (from-to)645-651
Number of pages7
JournalToxicologic Pathology
Volume27
Issue number6
DOIs
StatePublished - Nov 1999

Fingerprint

Uroplakins
Butylhydroxybutylnitrosamine
Rats
Carcinogenesis
Urothelium
Linings
Transitional Cell Carcinoma
Drinking Water
Carcinoma in Situ
Cells
Urinary Bladder
Inbred F344 Rats
Tumors
Membrane Proteins
Tissue
Staining and Labeling
Carcinoma

Keywords

  • Uroplakins
  • bladder cancer
  • cell membranes
  • differentiation

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Toxicology
  • Cell Biology

Cite this

Comparison of Uroplakin Expression During Urothelial Carcinogenesis Induced by N-Butyl-N-(4-Hydroxybutyl)Nitrosamine in Rats and Mice. / Ogawa, Kumiko; St. John, Margaret; De Oliveira, Maria Luiza; Arnold, Lora L; Shirai, Tomoyuki; Sun, Tung Tien; Cohen, Samuel Monroe.

In: Toxicologic Pathology, Vol. 27, No. 6, 11.1999, p. 645-651.

Research output: Contribution to journalArticle

Ogawa, Kumiko ; St. John, Margaret ; De Oliveira, Maria Luiza ; Arnold, Lora L ; Shirai, Tomoyuki ; Sun, Tung Tien ; Cohen, Samuel Monroe. / Comparison of Uroplakin Expression During Urothelial Carcinogenesis Induced by N-Butyl-N-(4-Hydroxybutyl)Nitrosamine in Rats and Mice. In: Toxicologic Pathology. 1999 ; Vol. 27, No. 6. pp. 645-651.
@article{2cffca2ce7c9459ebfdf455b964b11cf,
title = "Comparison of Uroplakin Expression During Urothelial Carcinogenesis Induced by N-Butyl-N-(4-Hydroxybutyl)Nitrosamine in Rats and Mice",
abstract = "The expression of uroplakins, the tissue-specific and differentiation-dependent membrane proteins of the urothelium, was analyzed immunohistochemically in N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN)-treated rats and mice during bladder carcinogenesis. Male Fischer 344 rats were treated with 0.05{\%} BBN in the drinking water for 10 wk and were euthanatized at week 20 of the experiment. BBN was administered to male B6D2F1 mice; it was either provided at a rate of 0.05{\%} in the drinking water (for 26 wk) or 5 mg BBN was administered by intragastric gavage twice weekly for 10 wk, followed by 20 wk without treatment. In rats, BBN-induced, noninvasive, low-grade, papillary, transitional cell carcinoma (TCC) showed decreased uroplakin-staining of cells lining the lumen but showed increased expression in some nonluminal cells. In mice, nonpapillary, high-grade dysplasia, carcinoma in situ, and invasive carcinoma were induced. There was a marked decrease in the number of uroplakin-positive cells lining the lumen and in nonluminal cells. This occurred in normal-appearing urothelium in BBN-treated mice and in dysplasic urothelium, in carcinoma in situ, and in invasive TCC. The percentage of uroplakin-positive nonluminal cells was higher in control mice than in rats, but it was lower in the mouse than in the rat after BBN treatment. Uroplakin expression was disorderly and focal in BBN-treated urothelium in both species. These results indicate that BBN treatment changed the expression of uroplakins during bladder carcinogenesis, with differences in rats and mice being related to degree of tumor differentiation.",
keywords = "Uroplakins, bladder cancer, cell membranes, differentiation",
author = "Kumiko Ogawa and {St. John}, Margaret and {De Oliveira}, {Maria Luiza} and Arnold, {Lora L} and Tomoyuki Shirai and Sun, {Tung Tien} and Cohen, {Samuel Monroe}",
year = "1999",
month = "11",
doi = "10.1177/019262339902700606",
language = "English (US)",
volume = "27",
pages = "645--651",
journal = "Toxicologic Pathology",
issn = "0192-6233",
publisher = "SAGE Publications Inc.",
number = "6",

}

TY - JOUR

T1 - Comparison of Uroplakin Expression During Urothelial Carcinogenesis Induced by N-Butyl-N-(4-Hydroxybutyl)Nitrosamine in Rats and Mice

AU - Ogawa, Kumiko

AU - St. John, Margaret

AU - De Oliveira, Maria Luiza

AU - Arnold, Lora L

AU - Shirai, Tomoyuki

AU - Sun, Tung Tien

AU - Cohen, Samuel Monroe

PY - 1999/11

Y1 - 1999/11

N2 - The expression of uroplakins, the tissue-specific and differentiation-dependent membrane proteins of the urothelium, was analyzed immunohistochemically in N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN)-treated rats and mice during bladder carcinogenesis. Male Fischer 344 rats were treated with 0.05% BBN in the drinking water for 10 wk and were euthanatized at week 20 of the experiment. BBN was administered to male B6D2F1 mice; it was either provided at a rate of 0.05% in the drinking water (for 26 wk) or 5 mg BBN was administered by intragastric gavage twice weekly for 10 wk, followed by 20 wk without treatment. In rats, BBN-induced, noninvasive, low-grade, papillary, transitional cell carcinoma (TCC) showed decreased uroplakin-staining of cells lining the lumen but showed increased expression in some nonluminal cells. In mice, nonpapillary, high-grade dysplasia, carcinoma in situ, and invasive carcinoma were induced. There was a marked decrease in the number of uroplakin-positive cells lining the lumen and in nonluminal cells. This occurred in normal-appearing urothelium in BBN-treated mice and in dysplasic urothelium, in carcinoma in situ, and in invasive TCC. The percentage of uroplakin-positive nonluminal cells was higher in control mice than in rats, but it was lower in the mouse than in the rat after BBN treatment. Uroplakin expression was disorderly and focal in BBN-treated urothelium in both species. These results indicate that BBN treatment changed the expression of uroplakins during bladder carcinogenesis, with differences in rats and mice being related to degree of tumor differentiation.

AB - The expression of uroplakins, the tissue-specific and differentiation-dependent membrane proteins of the urothelium, was analyzed immunohistochemically in N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN)-treated rats and mice during bladder carcinogenesis. Male Fischer 344 rats were treated with 0.05% BBN in the drinking water for 10 wk and were euthanatized at week 20 of the experiment. BBN was administered to male B6D2F1 mice; it was either provided at a rate of 0.05% in the drinking water (for 26 wk) or 5 mg BBN was administered by intragastric gavage twice weekly for 10 wk, followed by 20 wk without treatment. In rats, BBN-induced, noninvasive, low-grade, papillary, transitional cell carcinoma (TCC) showed decreased uroplakin-staining of cells lining the lumen but showed increased expression in some nonluminal cells. In mice, nonpapillary, high-grade dysplasia, carcinoma in situ, and invasive carcinoma were induced. There was a marked decrease in the number of uroplakin-positive cells lining the lumen and in nonluminal cells. This occurred in normal-appearing urothelium in BBN-treated mice and in dysplasic urothelium, in carcinoma in situ, and in invasive TCC. The percentage of uroplakin-positive nonluminal cells was higher in control mice than in rats, but it was lower in the mouse than in the rat after BBN treatment. Uroplakin expression was disorderly and focal in BBN-treated urothelium in both species. These results indicate that BBN treatment changed the expression of uroplakins during bladder carcinogenesis, with differences in rats and mice being related to degree of tumor differentiation.

KW - Uroplakins

KW - bladder cancer

KW - cell membranes

KW - differentiation

UR - http://www.scopus.com/inward/record.url?scp=0032722952&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032722952&partnerID=8YFLogxK

U2 - 10.1177/019262339902700606

DO - 10.1177/019262339902700606

M3 - Article

C2 - 10588545

AN - SCOPUS:0032722952

VL - 27

SP - 645

EP - 651

JO - Toxicologic Pathology

JF - Toxicologic Pathology

SN - 0192-6233

IS - 6

ER -