Comparison of traditional phenotypic identification methods with partial 5′ 16S rRNA gene sequencing for species-level identification of nonfermenting gram-negative bacilli

Joann L. Cloud, Dag Harmsen, Peter Charles Iwen, James J. Dunn, Gerri Hall, Paul Rocco Lasala, Karen Hoggan, Deborah Wilson, Gail L. Woods, Alexander Mellmann

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Abstract

Correct identification of nonfermenting Gram-negative bacilli (NFB) is crucial for patient management. We compared phenotypic identifications of 96 clinical NFB isolates with identifications obtained by 5′ 16S rRNA gene sequencing. Sequencing identified 88 isolates (91.7%) with >99% similarity to a sequence from the assigned species; 61.5% of sequencing results were concordant with phenotypic results, indicating the usability of sequencing to identify NFB.

Original languageEnglish (US)
Pages (from-to)1442-1444
Number of pages3
JournalJournal of clinical microbiology
Volume48
Issue number4
DOIs
Publication statusPublished - Apr 1 2010

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ASJC Scopus subject areas

  • Microbiology (medical)

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