Comparison of the signaling and stability of electrochemical DNA sensors fabricated from 6- or 11-carbon self-assembled monolayers

Rebecca Y. Lai, Dwight S. Seferos, Alan J. Heeger, Guillermo C. Bazan, Kevin W. Plaxco

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

We have characterized the solution-phase and dry storage stability of electrochemical E-DNA sensors fabricated using mixed self-assembled monolayers (SAMs) composed of 6- or 11 -carbon (C6 and C11, respectively) α,ω-thiol alcohols and the analogous C6- or C11-thiol-terminated stem-loop DNA probe. We find that the solution-phase and dry storage stability of C6-based E-DNA sensors are limited and poorly reproducible. The use of stabilizing agents bovine serum albumin plus either glucose or trehalose significantly improves the dry storage shelf life of such sensors: when using these preservatives, we observe only 7-9% sensor degradation after 1 month of storage in air at room temperature. In comparison, the stability of C11-based E-DNA sensors is significantly greater than that of the C6-based sensors; we observe only minor (5-8%) loss of signal upon storing these sensors for a week under ambient solution conditions or for more than a month in air in the presence of preservatives. Moreover, whereas the electron-transfer rate through C11 SAMs is slower than that observed for C6 SAMs, it is rapid enough to support good sensor performance. It thus appears that C11 SAMs provide a reasonable compromise between electron-transfer efficiency and sensor stability and are well suited for use in electronic DNA-sensing applications.

Original languageEnglish (US)
Pages (from-to)10796-10800
Number of pages5
JournalLangmuir
Volume22
Issue number25
DOIs
StatePublished - Dec 5 2006

Fingerprint

Self assembled monolayers
DNA
Carbon
deoxyribonucleic acid
carbon
sensors
Sensors
preservatives
storage stability
Sulfhydryl Compounds
thiols
electron transfer
Trehalose
Electrons
Excipients
air
DNA Probes
Bovine Serum Albumin
Air
albumins

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Comparison of the signaling and stability of electrochemical DNA sensors fabricated from 6- or 11-carbon self-assembled monolayers. / Lai, Rebecca Y.; Seferos, Dwight S.; Heeger, Alan J.; Bazan, Guillermo C.; Plaxco, Kevin W.

In: Langmuir, Vol. 22, No. 25, 05.12.2006, p. 10796-10800.

Research output: Contribution to journalArticle

Lai, Rebecca Y. ; Seferos, Dwight S. ; Heeger, Alan J. ; Bazan, Guillermo C. ; Plaxco, Kevin W. / Comparison of the signaling and stability of electrochemical DNA sensors fabricated from 6- or 11-carbon self-assembled monolayers. In: Langmuir. 2006 ; Vol. 22, No. 25. pp. 10796-10800.
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